dc.contributor.authorMa, Xing
dc.contributor.authorDevi, Gitali
dc.contributor.authorQu, Qiuyu
dc.contributor.authorToh, Desiree-Faye Kaixin
dc.contributor.authorChen, Gang
dc.contributor.authorZhao, Yanli
dc.identifier.citationMa, X., Devi, G., Qu, Q., Toh, D.-F. K., Chen, G., & Zhao, Y. (2014). Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles. Bioconjugate chemistry, 25(8), 1412-1420.en_US
dc.description.abstractIn order to overcome poor cell permeability of antisense peptide nucleic acid (PNA), a fluorescent mesoporous silica nanoparticle (MSNP) carrier was developed to successfully deliver antisense PNA into cancer cells for effective silence of B-cell lymphoma 2 (Bcl-2) protein expression in vitro. First, fluorescent MSNP functionalized with disulfide bond bridged groups was fabricated and characterized. Antisense and negative control PNAs were synthesized and further conjugated with fluorescent dye cyanine 5. Then, the PNAs were covalently connected with fluorescent MSNP via amidation between amino group of PNAs and carboxylic acid group on the MSNP surface. High intracellular concentration of glutathione serves as a natural reducing agent, which could cleave the disulfide bond to trigger the PNA release in vitro. Confocal laser scanning microscopy studies prove that PNA conjugated MSNP was endocytosed by HeLa cancer cells, and redox-controlled intracellular release of antisense PNA from fluorescent MSNP was successfully achieved. Finally, effective silencing of the Bcl-2 protein expression induced by the delivered antisense PNA into HeLa cells was confirmed by Western blot assay.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent29 p.en_US
dc.relation.ispartofseriesBioconjugate chemistryen_US
dc.rights© 2014 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Bioconjugate Chemistry, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/bc5002714].en_US
dc.titleIntracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticlesen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.description.versionAccepted versionen_US

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