Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/104006
Title: Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection
Authors: Cerny, Daniela
Haniffa, Muzlifah
Shin, Amanda
Bigliardi, Paul
Tan, Bien Keem
Lee, Bernett
Poidinger, Michael
Tan, Ern Yu
Ginhoux, Florent
Fink, Katja
Keywords: DRNTU::Science::Biological sciences::Microbiology::Bacteria
Issue Date: 2014
Source: Cerny, D., Haniffa, M., Shin, A., Bigliardi, P., Tan, B. K., Lee, B., et al. (2014). Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection. PLoS pathogens, 10(12), e1004548-.
Series/Report no.: PLoS pathogens
Abstract: Dengue is a growing global concern with 390 million people infected each year. Dengue virus (DENV) is transmitted by mosquitoes, thus host cells in the skin are the first point of contact with the virus. Human skin contains several populations of antigen-presenting cells which could drive the immune response to DENV in vivo: epidermal Langerhans cells (LCs), three populations of dermal dendritic cells (DCs), and macrophages. Using samples of normal human skin we detected productive infection of CD14+ and CD1c+ DCs, LCs and dermal macrophages, which was independent of DC-SIGN expression. LCs produced the highest viral titers and were less sensitive to IFN-β. Nanostring gene expression data showed significant up-regulation of IFN-β, STAT-1 and CCL5 upon viral exposure in susceptible DC populations. In mice infected intra-dermally with DENV we detected parallel populations of infected DCs originating from the dermis and migrating to the skin-draining lymph nodes. Therefore dermal DCs may simultaneously facilitate systemic spread of DENV and initiate the adaptive anti-viral immune response.
URI: https://hdl.handle.net/10356/104006
http://hdl.handle.net/10220/24602
ISSN: 1553-7374
DOI: 10.1371/journal.ppat.1004548
Rights: © 2014 Cerny et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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