Injectable, interconnected, high-porosity macroporous biocompatible gelatin scaffolds made by surfactant-free emulsion templating
Oh, Bernice H. L.
Chan-Park, Mary B.
Date of Issue2014
School of Chemical and Biomedical Engineering
High-porosity interconnected, thermoresponsive macroporous hydrogels are prepared from oil-in-water high internal phase emulsions (HIPEs) stabilized by gelatin-graft-poly(N-isopropylacrylamide). PolyHIPEs are obtained by gelling HIPEs utilizing the thermoresponsiveness of the copolymer components. PolyHIPEs properties can be controlled by varying the aqueous phase composition, internal phase volume ratio, and gelation temperature. PolyHIPEs respond to temperature changes experienced during cell seeding, allowing fibroblasts to spread, proliferate, and penetrate into the scaffold. Encapsulated cells survive ejection of cell-laden hydrogels through a hypodermic needle. This system provides a new strategy for the fabrication of safe injectable biocompatible tissue engineering scaffolds.
DRNTU::Science::Biological sciences::Molecular biology
Macromolecular rapid communications
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.