Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip
Date of Issue2014
School of Chemical and Biomedical Engineering
A microfluidic chip integrated with pneumatically controlled valves was developed for multiplexed biomolecular detection via localized surface plasmonic resonance (LSPR) of single gold nanorod. The cost-effective microfluidic chip was assembled by polydimethylsiloxane (PDMS) layers and glass substrates with a precisely controlled thickness. The thin and flat microfluidic chip fitted the narrow space of dark-field microscopy and enabled the recording of single nanoparticle LSPR responses. Aptamer-antigen-antibody sandwiched detection scheme was utilized to enhance the LSPR responses for label-free biomolecular detection. This microfluidic chip successfully demonstrated the multiplexed detection of three independent analytes (PSA, IgE, and thrombin).
DRNTU::Engineering::Chemical engineering::Biochemical engineering
© 2014 Springer Science+Business Media New York. This is the author created version of a work that has been peer reviewed and accepted for publication by Plasmonics, Springer Science+Business Media New York. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [Article DOI: http://dx.doi.org/10.1007/s11468-013-9661-4].