A neuroprotective role for microRNA miR-1000 mediated by limiting glutamate excitotoxicity
Augustine, George J
Cohen, Stephen M
Date of Issue2015
Lee Kong Chian School of Medicine
Evidence has begun to emerge for microRNAs as regulators of synaptic signaling, specifically acting to control postsynaptic responsiveness during synaptic transmission. In this report, we provide evidence that Drosophila melanogaster miR-1000 acts presynaptically to regulate glutamate release at the synapse by controlling expression of the vesicular glutamate transporter (VGlut). Genetic deletion of miR-1000 led to elevated apoptosis in the brain as a result of glutamatergic excitotoxicity. The seed-similar miR-137 regulated VGluT2 expression in mouse neurons. These conserved miRNAs share a neuroprotective function in the brains of flies and mice. Drosophila miR-1000 showed activity-dependent expression, which might serve as a mechanism to allow neuronal activity to fine-tune the strength of excitatory synaptic transmission.
DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology
© 2015 The Author(s) (Nature Publishing Group). This is the author created version of a work that has been peer reviewed and accepted for publication by Nature Neuroscience, The Author(s) (Nature Publishing Group). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1038/nn.3935].