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      Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by a new bacterial strain Pseudomonas putida NBUS12

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      2015_Bioconversion of Styrene to Poly(hydroxyalkanoate) (PHA) by the New Bacterial Strain Pseudomonas putida NBUS12.pdf (933.8Kb)
      Author
      Tan, Giin-Yu Amy
      Chen, Chia-Lung
      Ge, Liya
      Li, Ling
      Tan, Swee Ngin
      Wang, Jing-Yuan
      Date of Issue
      2015
      School
      School of Civil and Environmental Engineering
      Research Centre
      Nanyang Environment and Water Research Institute
      Related Organization
      Natural Sciences and Science Education Academic Group
      Version
      Published version
      Abstract
      Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer medium-chain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05±0.00 and 23.10±3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a genetically-distinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28±0.10 g L−1 and PHA content of 32.49±2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C6 ), 3-hydroxyoctanoate (C8 ), 3-hydroxydecanoate (C10 ), 3-hydroxydodecanoate (C12 ), and 3-hydroxytetradecanoate (C14 ) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.
      Subject
      DRNTU::Science::Biological sciences::Microbiology::Bacteria
      Type
      Journal Article
      Series/Journal Title
      Microbes and environments
      Rights
      © 2015 Japanese Society of Microbial Ecology. This paper was published in Microbes and Environments and is made available as an electronic reprint (preprint) with permission of Japanese Society of Microbial Ecology. The paper can be found at the following official DOI: [http://dx.doi.org/10.1264/jsme2.ME14138]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
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      http://dx.doi.org/10.1264/jsme2.ME14138
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