Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/99785
Title: Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein
Authors: Li, Yan
Jain, Neeraj
Limpanawat, Suweeraya
To, Janet
Quistgaard, Esben M.
Nordlund, Par
Thanabalu, Thirumaran
Torres, Jaume
Keywords: DRNTU::Science::Biological sciences::Human anatomy and physiology
Issue Date: 2015
Source: Li, Y., Jain, N., Limpanawat, S., To, J., Quistgaard, E. M., Nordlund, P., et al. (2015). Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Virology, 482, 105-110.
Series/Report no.: Virology
Abstract: The small hydrophobic (SH) protein is a short channel-forming polypeptide encoded by the human respiratory syncytial virus (hRSV). Deletion of SH protein leads to the viral attenuation in mice and primates, and delayed apoptosis in infected cells. We have used a membrane-based yeast two-hybrid system (MbY2H) and a library from human lung cDNA to detect proteins that bind SH protein. This led to the identification of a membrane protein, B-cell associated protein 31 (BAP31). Transfected SH protein co-localizes with transfected BAP31 in cells, and pulls down endogenous BAP31. Titration of purified C-terminal endodomain of BAP31 against isotopically labeled SH protein in detergent micelles suggests direct interaction between the two proteins. Given the key role of BAP31 in protein trafficking and its critical involvement in pro- and anti-apoptotic pathways, this novel interaction may constitute a potential drug target.
URI: https://hdl.handle.net/10356/99785
http://hdl.handle.net/10220/25675
ISSN: 0042-6822
DOI: 10.1016/j.virol.2015.03.034
Rights: © 2015 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Virology, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.virol.2015.03.034].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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