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      MS2 virus inactivation by atmospheric-pressure cold plasma using different gas carriers and power levels

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      MS2 Virus Inactivation by Atmospheric-Pressure Cold Plasma Using.pdf (1.175Mb)
      Author
      Wu, Yan
      Liang, Yongdong
      Wei, Kai
      Li, Wei
      Yao, Maosheng
      Zhang, Jue
      Grinshpun, Sergey A.
      Date of Issue
      2015
      School
      School of Civil and Environmental Engineering
      Version
      Published version
      Abstract
      In this study, airborne MS2 bacteriophages were exposed for subsecond time intervals to atmospheric-pressure cold plasma (APCP) produced using different power levels (20, 24, and 28 W) and gas carriers (ambient air, Ar-O2 [2%, vol/vol], and He-O2 [2%, vol/vol]). In addition, waterborne MS2 viruses were directly subjected to the APCP treatment for up to 3 min. MS2 viruses with and without the APCP exposure were examined by scanning electron microscopy (SEM), reverse transcription-PCR (RT-PCR), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Viral inactivation was shown to exhibit linear relationships with the APCP generation power and exposure time (R(2) > 0.95 for all energy levels tested) up to 95% inactivation (1.3-log reduction) after a subsecond airborne exposure at 28 W; about the same inactivation level was achieved for waterborne viruses with an exposure time of less than 1 min. A larger amount of reactive oxygen species (ROS), such as atomic oxygen, in APCP was detected for a higher generation power with Ar-O2 and He-O2 gas carriers. SEM images, SDS-PAGE, and agarose gel analysis of exposed waterborne viruses showed various levels of damage to both surface proteins and their related RNA genes after the APCP exposure, thus leading to the loss of their viability and infectivity.
      Subject
      DRNTU::Science::Biological sciences::Microbiology
      Type
      Journal Article
      Series/Journal Title
      Applied and environmental microbiology
      Rights
      © 2015 American Society for Microbiology. This paper was published in Applied and Environmental Microbiology and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology (ASM). The paper can be found at the following official DOI: [http://dx.doi.org/10.1128/AEM.03322-14]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
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      http://dx.doi.org/10.1128/AEM.03322-14
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