dc.contributor.authorChiang, Wen-Chi
dc.contributor.authorNilsson, Martin
dc.contributor.authorJensen, Peter Østrup
dc.contributor.authorHøiby, Niels
dc.contributor.authorNielsen, Thomas Eiland
dc.contributor.authorGivskov, Michael
dc.contributor.authorTolker-Nielsen, Tim
dc.identifier.citationChiang, W.-C., Nilsson, M., Jensen, P. Ø., Høiby, N., Nielsen, T. E., Givskov, M., et al. (2013). Extracellular DNA shields against aminoglycosides in pseudomonas aeruginosa biofilms. Antimicrobial agents and chemotherapy, 57(5), 2352-2361.en_US
dc.description.abstractWithin recent years, it has been established that extracellular DNA is a key constituent of the matrix of microbial biofilms. In addition, it has recently been demonstrated that DNA binds positively charged antimicrobials such as aminoglycosides and antimicrobial peptides. In the present study, we provide evidence that extracellular DNA shields against aminoglycosides in Pseudomonas aeruginosa biofilms. We show that exogenously supplemented DNA integrates into P. aeruginosa biofilms and increases their tolerance toward aminoglycosides. We provide evidence that biofilms formed by a DNA release-deficient P. aeruginosa quorum-sensing mutant are more susceptible to aminoglycoside treatment than wild-type biofilms but become rescued from the detrimental action of aminoglycosides upon supplementation with exogenous DNA. Furthermore, we demonstrate that exposure to lysed polymorphonuclear leukocytes, which are thought to be a source of extracellular DNA at sites of infections, increases the tolerance of P. aeruginosa biofilms toward aminoglycosides. Although biofilm-associated aminoglycoside tolerance recently has been linked to extracellular DNA-mediated activation of the pmr genes, we demonstrate that the aminoglycoside tolerance mediated by the presence of extracellular DNA is not caused by activation of the pmr genes in our P. aeruginosa biofilms but rather by a protective shield effect of the extracellular DNA.en_US
dc.relation.ispartofseriesAntimicrobial agents and chemotherapyen_US
dc.rights© 2013 American Society for Microbiology (ASM). This paper was published in Antimicrobial Agents and Chemotherapy and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology (ASM). The paper can be found at the following official DOI: [http://dx.doi.org/10.1128/AAC.00001-13]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en_US
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Bacteria
dc.titleExtracellular DNA shields against aminoglycosides in pseudomonas aeruginosa biofilmsen_US
dc.typeJournal Article
dc.description.versionPublished versionen_US
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen_US

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