dc.contributor.authorEvrard, Maximilien
dc.contributor.authorChong, Shu Zhen
dc.contributor.authorDevi, Sapna
dc.contributor.authorChew, Weng Keong
dc.contributor.authorLee, Bernett
dc.contributor.authorPoidinger, Michael
dc.contributor.authorGinhoux, Florent
dc.contributor.authorTan, Suet Mien
dc.contributor.authorNg, Lai Guan
dc.date.accessioned2015-06-16T10:19:23Z
dc.date.available2015-06-16T10:19:23Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.identifier.citationEvrard, M., Chong, S. Z., Devi, S., Chew, W. K., Lee, B., Poidinger, M., et al. (2015). Visualization of bone marrow monocyte mobilization using Cx3cr1gfp/+Flt3L-/- reporter mouse by multiphoton intravital microscopy. Journal of leukocyte biology, 97(3), 611-619.en_US
dc.identifier.issn0741-5400en_US
dc.identifier.urihttp://hdl.handle.net/10220/25919
dc.description.abstractMonocytes are innate immune cells that play critical roles in inflammation and immune defense. A better comprehension of how monocytes are mobilized and recruited is fundamental to understand their biologic role in disease and steady state. The BM represents a major “checkpoint” for monocyte homeostasis, as it is the primary site for their production and release. Our study determined that the Cx3cr1gfp/+ mouse strain is currently the most ideal model for the visualization of monocyte behavior in the BM by multiphoton intravital microscopy. However, we observed that DCs are also labeled with high levels of GFP and thus, interfere with the accuracy of monocyte tracking in vivo. Hence, we generated a Cx3cr1gfp/+Flt3L−/− reporter mouse and showed that whereas monocyte numbers were not affected, DC numbers were reduced significantly, as DCs but not monocytes depend on Flt3 signaling for their development. We thus verified that mobilization of monocytes from the BM in Cx3cr1gfp/+Flt3L−/− mice is intact in response to LPS. Collectively, our study demonstrates that the Cx3cr1gfp/+Flt3L−/− reporter mouse model represents a powerful tool to visualize monocyte activities in BM and illustrates the potential of a Cx3cr1gfp/+-based, multifunctionality fluorescence reporter approach to dissect monocyte function in vivo.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesJournal of leukocyte biologyen_US
dc.rights© 2015 Society for Leukocyte Biology (SLB).en_US
dc.subjectDRNTU::Science::Biological sciences
dc.titleVisualization of bone marrow monocyte mobilization using Cx3cr1gfp/+Flt3L-/- reporter mouse by multiphoton intravital microscopyen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1189/jlb.1TA0514-274R


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