dc.contributor.authorGiannakakis, Antonis
dc.contributor.authorZhang, Jingxian
dc.contributor.authorJenjaroenpun, Piroon
dc.contributor.authorNama, Srikanth
dc.contributor.authorZainolabidin, Norliyana
dc.contributor.authorAau, Mei Yee
dc.contributor.authorYarmishyn, Aliaksandr A.
dc.contributor.authorVaz, Candida
dc.contributor.authorIvshina, Anna Vladimirovna
dc.contributor.authorGrinchuk, Oleg V.
dc.contributor.authorVoorhoeve, Mathijs
dc.contributor.authorVardy, Leah Anne
dc.contributor.authorSampath, Prabha
dc.contributor.authorKuznetsov, Vladimir Andreevich
dc.contributor.authorKurochkin, Igor V.
dc.contributor.authorGuccione, Ernesto
dc.date.accessioned2015-06-25T01:36:03Z
dc.date.available2015-06-25T01:36:03Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.identifier.citationGiannakakis, A., Zhang, J., Jenjaroenpun, P., Nama, S., Zainolabidin, N., Aau, M. Y., et al. (2015). Contrasting expression patterns of coding and noncoding parts of the human genome upon oxidative stress. Scientific Reports, 5, 9737-.en_US
dc.identifier.issn2045-2322en_US
dc.identifier.urihttp://hdl.handle.net/10220/26058
dc.description.abstractOxidative stress (OS) is caused by an imbalance between pro- and anti-oxidant reactions leading to accumulation of reactive oxygen species within cells. We here investigate the effect of OS on the transcriptome of human fibroblasts. OS causes a rapid and transient global induction of transcription characterized by pausing of RNA polymerase II (PolII) in both directions, at specific promoters, within 30 minutes of the OS response. In contrast to protein-coding genes, which are commonly down-regulated, this novel divergent, PolII pausing-phenomenon leads to the generation of thousands of long noncoding RNAs (lncRNAs) with promoter-associated antisense lncRNAs transcripts (si-paancRNAs) representing the major group of stress-induced transcripts. OS causes transient dynamics of si-lncRNAs in nucleus and cytosol, leading to their accumulation at polysomes, in contrast to mRNAs, which get depleted from polysomes. We propose that si-lncRNAs represent a novel component of the transcriptional stress that is known to determine the outcome of immediate-early and later cellular stress responses and we provide insights on the fate of those novel mature lncRNA transcripts by showing that their association with polysomal complexes is significantly increased in OS.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.language.isoenen_US
dc.relation.ispartofseriesScientific Reportsen_US
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
dc.subjectDRNTU::Science::Biological sciences::Genetics
dc.titleContrasting expression patterns of coding and noncoding parts of the human genome upon oxidative stressen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Computer Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1038/srep09737
dc.description.versionPublished versionen_US


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