dc.contributor.authorMatysik, Artur
dc.contributor.authorKraut, Rachel Susan
dc.date.accessioned2015-07-15T09:16:29Z
dc.date.available2015-07-15T09:16:29Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.identifier.citationMatysik, A., & Kraut, R. S. (2014). Preparation of mica supported lipid bilayers for high resolution optical microscopy imaging. Journal of Visualized Experiments, 88, e52054.en_US
dc.identifier.issn1940-087Xen_US
dc.identifier.urihttp://hdl.handle.net/10220/38341
dc.description.abstractSupported lipid bilayers (SLBs) are widely used as a model for studying membrane properties (phase separation, clustering, dynamics) and its interaction with other compounds, such as drugs or peptides. However SLB characteristics differ depending on the support used. Commonly used techniques for SLB imaging and measurements are single molecule fluorescence microscopy, FCS and atomic force microscopy (AFM). Because most optical imaging studies are carried out on a glass support, while AFM requires an extremely flat surface (generally mica), results from these techniques cannot be compared directly, since the charge and smoothness properties of these materials strongly influence diffusion. Unfortunately, the high level of manual dexterity required for the cutting and gluing thin slices of mica to the glass slide presents a hurdle to routine use of mica for SLB preparation. Although this would be the method of choice, such prepared mica surfaces often end up being uneven (wavy) and difficult to image, especially with small working distance, high numerical aperture lenses. Here we present a simple and reproducible method for preparing thin, flat mica surfaces for lipid vesicle deposition and SLB preparation. Additionally, our custom made chamber requires only very small volumes of vesicles for SLB formation. The overall procedure results in the efficient, simple and inexpensive production of high quality lipid bilayer surfaces that are directly comparable to those used in AFM studies.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesJournal of visualized experimentsen_US
dc.rights© 2014 Journal of Visualized Experiments. This paper was published in Journal of Visualized Experiments and is made available as an electronic reprint (preprint) with permission of Journal of Visualized Experiments. The published version is available at: [http://dx.doi.org/10.3791/52054]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en_US
dc.subjectDRNTU::Science::Biological sciences::Microbiology::Microbial ecology
dc.titlePreparation of mica supported lipid bilayers for high resolution optical microscopy imagingen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.3791/52054
dc.description.versionPublished versionen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record