Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/97014
Title: Characterization of a novel endoglucanase from Ganoderma lucidum
Authors: Manavalan, Tamilvendan
Manavalan, Arulmani
Thangavelu, Kalaichelvan P.
Heese, Klaus
Keywords: DRNTU::Science::Biological sciences::Microbiology
Issue Date: 2015
Source: Manavalan, T., Manavalan, A., Thangavelu, K. P., & Heese, K. (2015). Characterization of a novel endoglucanase from Ganoderma lucidum. Journal of Basic Microbiology, 55(6), 761-771.
Series/Report no.: Journal of basic microbiology
Abstract: We evaluated the production and characterization of endoglucanase from Ganoderma lucidum using different lignocellulose biomasses. We purified a novel carboxymethyl cellulose (CMC) hydrolyzing endoglucanase from the white-rot fungus G. lucidum when the medium was supplemented with 1% (w/v) wheat bran. Endoglucanase was purified 12.5-fold via ammonium sulfate fractionation, Sephadex G-100, and Q-Sepharose column chromatography with a final yield of 15%. SDS-PAGE analysis revealed that the endoglucanase had a molecular mass of 64.0 kDa. The optimal activity of purified endoglucanase was at pH 5.0 and 35 °C, though it was stable between pH 4.0–7.0 and temperatures of 30–60 °C. The purified enzyme was specific to CMC as a suitable substrate. The metal ions Hg2+, Fe2+, and Cr2+ inhibited enzyme activity, while Ca2+, Mg2+, and Mn2+ enhanced enzyme activity. The endoglucanase showed high activity and stability in the presence of different surfactants and non-polar hydrophobic organic solvents. This endoglucanase is tolerant to high temperature, metal ions, surfactants, and solvents, suggesting that it is appropriate for use in biomass conversion for biofuel production under harsh environmental conditions.
URI: https://hdl.handle.net/10356/97014
http://hdl.handle.net/10220/38508
ISSN: 0233-111X
DOI: 10.1002/jobm.201400808
Rights: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:IAS Journal Articles
SBS Journal Articles

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.