dc.contributor.authorKoh, Esther Y. C.
dc.contributor.authorHo, Steven C. L.
dc.contributor.authorSong, Zhiwei
dc.contributor.authorBi, Xuezhi
dc.contributor.authorBardor, Muriel
dc.contributor.authorYang, Yuansheng
dc.contributor.editorHendershot, Linda M.*
dc.identifier.citationKoh, E. Y. C., Ho, S. C. L., Mariati , Song, Z., Bi, X., Bardor, M., et al. (2013). An internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cells. PLoS One, 8(12), e82100-.en_US
dc.description.abstractA set of mutated Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) elements with varying strengths is generated by mutating the translation initiation codons of 10th, 11th, and 12th AUG to non-AUG triplets. They are able to control the relative expression of multiple genes over a wide range in mammalian cells in both transient and stable transfections. The relative strength of each IRES mutant remains similar in different mammalian cell lines and is not gene specific. The expressed proteins have correct molecular weights. Optimization of light chain over heavy chain expression by these IRES mutants enhances monoclonal antibody expression level and quality in stable transfections. Uses of this set of IRES mutants can be extended to other applications such as synthetic biology, investigating interactions between proteins and its complexes, cell engineering, multi-subunit protein production, gene therapy, and reprogramming of somatic cells into stem cells.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)
dc.relation.ispartofseriesPLoS Oneen_US
dc.rights© 2013 Koh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.titleAn internal ribosome entry site (IRES) mutant library for tuning expression level of multiple genes in mammalian cellsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.description.versionPublished versionen_US

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