dc.contributor.authorHartrianti, Pietradewi
dc.contributor.authorLing, Ling
dc.contributor.authorGoh, Lyn Mei Ming
dc.contributor.authorOw, Kok Seng Amos
dc.contributor.authorSamsonraj, Rebekah Margaret
dc.contributor.authorSow, Wan Ting
dc.contributor.authorWang, Shuai
dc.contributor.authorNurcombe, Victor
dc.contributor.authorCool, Simon M.
dc.contributor.authorNg, Kee Woei
dc.date.accessioned2015-09-03T06:30:39Z
dc.date.available2015-09-03T06:30:39Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.identifier.citationHartrianti, P., Ling, L., Goh, L. M. M., Ow, K. S. A., Samsonraj, R. M., Sow, W. T., et al. (2015). Modulating mesenchymal stem cell behavior using human hair keratin-coated surfaces. Stem Cells International, 2015, 752424-.en_US
dc.identifier.urihttp://hdl.handle.net/10220/38559
dc.description.abstractHuman mesenchymal stem cells (hMSCs) have shown great potential for therapeutic purposes. However, the low frequencies of hMSCs in the body and difficulties in expanding their numbers in vitro have limited their clinical use. In order to develop an alternative strategy for the expansion of hMSCs in vitro, we coated tissue culture polystyrene with keratins extracted from human hair and studied the behavior of cells from 2 donors on these surfaces. The coating resulted in a homogeneous distribution of nanosized keratin globules possessing significant hydrophilicity. Results from cell attachment assays demonstrated that keratincoated surfaces were able to moderate donor-to-donor variability when compared with noncoated tissue culture polystyrene. STRO-1 expression was either sustained or enhanced on hMSCs cultured on keratin-coated surfaces.This translated into significant increases in the colony-forming efficiencies of both hMSC populations, when the cells were serially passaged. Human hair keratins are abundant and might constitute a feasible replacement for other biomaterials that are of animal origin. In addition, our results suggest that hair keratins may be effective in moderating the microenvironment sufficiently to enrich hMSCs with high colonyforming efficiency ex vivo, for clinical applications.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)
dc.format.extent10 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesStem Cells Internationalen_US
dc.rights© 2015 Pietradewi Hartrianti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectDRNTU::Science::Biological sciences::Human anatomy and physiology
dc.titleModulating mesenchymal stem cell behavior using human hair keratin-coated surfacesen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1155/2015/752424
dc.description.versionPublished versionen_US


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