dc.contributor.authorAmaladoss, Anburaj
dc.contributor.authorChen, Qingfeng
dc.contributor.authorLiu, Min
dc.contributor.authorDummler, Sara K.
dc.contributor.authorDao, Ming
dc.contributor.authorSuresh, Subra
dc.contributor.authorChen, Jianzhu
dc.contributor.authorPreiser, Peter R.
dc.contributor.editorMarinho, Claudio Romero Farias*
dc.date.accessioned2015-09-17T05:28:42Z
dc.date.available2015-09-17T05:28:42Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.identifier.citationAmaladoss, A., Chen, Q., Liu, M., Dummler, S. K., Dao, M., Suresh, S., et al. (2015). De Novo Generated Human Red Blood Cells in Humanized Mice Support Plasmodium falciparum Infection. PLOS ONE, 10(6), e0129825-.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10220/38703
dc.description.abstractImmunodeficient mouse–human chimeras provide a powerful approach to study host specific pathogens like Plasmodium (P.) falciparum that causes human malaria. Existing mouse models of P. falciparum infection require repeated injections of human red blood cells (RBCs). In addition, clodronate lipsomes and anti-neutrophil antibodies are injected to suppress the clearance of human RBCs by the residual immune system of the immunodeficient mice. Engraftment of NOD-scid Il2rg-/- mice with human hematopoietic stem cells leads to reconstitution of human immune cells. Although human B cell reconstitution is robust and T cell reconstitution is reasonable in the recipient mice, human RBC reconstitution is generally poor or undetectable. The poor reconstitution is mainly the result of a deficiency of appropriate human cytokines that are necessary for the development and maintenance of these cell lineages. Delivery of plasmid DNA encoding human erythropoietin and interleukin-3 into humanized mice by hydrodynamic tail-vein injection resulted in significantly enhanced reconstitution of erythrocytes. With this improved humanized mouse, here we show that P. falciparum infects de novo generated human RBCs, develops into schizonts and causes successive reinvasion. We also show that different parasite strains exhibit variation in their ability to infect these humanized mice. Parasites could be detected by nested PCR in the blood samples of humanized mice infected with P. falciparum K1 and HB3 strains for 3 cycles, whereas in other strains such as 3D7, DD2, 7G8, FCR3 and W2mef parasites could only be detected for 1 cycle. In vivo adaptation of K1 strain further improves the infection efficiency and parasites can be detected by microscopy for 3 cycles. The parasitemia ranges between 0.13 and 0.25% at the first cycle of infection, falls between 0.08 and 0.15% at the second cycle, and drops to barely detectable levels at the third cycle of infection. Compared to existing mouse models, our model generates human RBCs de novo and does not require the treatment of mice with immunomodulators.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesPLOS ONEen_US
dc.rights© 2015 Amaladoss et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden_US
dc.titleDe Novo Generated Human Red Blood Cells in Humanized Mice Support Plasmodium falciparum Infectionen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0129825
dc.description.versionPublished versionen_US


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