Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/79270
Title: Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor
Authors: Wang, Victor Bochuan
Sivakumar, Krishnakumar
Yang, Liang
Zhang, Qichun
Kjelleberg, Staffan
Loo, Say Chye Joachim
Cao, Bin
Issue Date: 2015
Source: Wang, V. B., Sivakumar, K., Yang, L., Zhang, Q., Kjelleberg, S., Loo, S. C. J., et al. (2015). Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor. Scientific Reports, 5, 11222-.
Series/Report no.: Scientific Reports
Abstract: Mutualistic interactions in planktonic microbial communities have been extensively studied. However, our understanding on mutualistic communities consisting of co-existing planktonic cells and biofilms is limited. Here, we report a planktonic cells-biofilm mutualistic system established by the fermentative bacterium Escherichia coli and the dissimilatory metal-reducing bacterium Shewanella oneidensis in a bioelectrochemical device, where planktonic cells in the anode media interact with the biofilms on the electrode. Our results show that the transfer of formate is the key mechanism in this mutualistic system. More importantly, we demonstrate that the relative distribution of E. coli and S. oneidensis in the liquid media and biofilm is likely driven by their metabolic functions towards an optimum communal metabolism in the bioelectrochemical device. RNA sequencing-based transcriptomic analyses of the interacting organisms in the mutualistic system potentially reveal differential expression of genes involved in extracellular electron transfer pathways in both species in the planktonic cultures and biofilms.
URI: https://hdl.handle.net/10356/79270
http://hdl.handle.net/10220/38735
ISSN: 2045-2322
DOI: http://dx.doi.org/10.1038/srep11222
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:CEE Journal Articles
MSE Journal Articles
SBS Journal Articles
SCELSE Journal Articles

Google ScholarTM

Check

Altmetric

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.