dc.contributor.authorFook Kong, Tian
dc.contributor.authorYe, Weijian
dc.contributor.authorPeng, Weng Kung
dc.contributor.authorHou, Han Wei
dc.contributor.authorPreiser, Peter Rainer
dc.contributor.authorNguyen, Nam-Trung
dc.contributor.authorHan, Jongyoon
dc.identifier.citationFook Kong, T., Ye, W., Peng, W. K., Hou, H. W., Marcos, Preiser, P. R., et al. (2015). Enhancing malaria diagnosis through microfluidic cell enrichment and magnetic resonance relaxometry detection. Scientific Reports, 5, 11425-.en_US
dc.description.abstractDespite significant advancements over the years, there remains an urgent need for low cost diagnostic approaches that allow for rapid, reliable and sensitive detection of malaria parasites in clinical samples. Our previous work has shown that magnetic resonance relaxometry (MRR) is a potentially highly sensitive tool for malaria diagnosis. A key challenge for making MRR based malaria diagnostics suitable for clinical testing is the fact that MRR baseline fluctuation exists between individuals, making it difficult to detect low level parasitemia. To overcome this problem, it is important to establish the MRR baseline of each individual while having the ability to reliably determine any changes that are caused by the infection of malaria parasite. Here we show that an approach that combines the use of microfluidic cell enrichment with a saponin lysis before MRR detection can overcome these challenges and provide the basis for a highly sensitive and reliable diagnostic approach of malaria parasites. Importantly, as little as 0.0005% of ring stage parasites can be detected reliably, making this ideally suited for the detection of malaria parasites in peripheral blood obtained from patients. The approaches used here are envisaged to provide a new malaria diagnosis solution in the near future.en_US
dc.relation.ispartofseriesScientific Reportsen_US
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
dc.titleEnhancing malaria diagnosis through microfluidic cell enrichment and magnetic resonance relaxometry detectionen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.schoolLee Kong Chian School of Medicine
dc.description.versionPublished versionen_US

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