dc.contributor.authorYeo, David
dc.contributor.authorWiraja, Christian
dc.contributor.authorChuah, Yon Jin
dc.contributor.authorGao, Yu
dc.contributor.authorXu, Chenjie
dc.identifier.citationYeo, D., Wiraja, C., Chuah, Y. J., Gao, Y., & Xu, C. (2015). A Nanoparticle-based Sensor Platform for Cell Tracking and Status/Function Assessment. Scientific Reports, 5, 14768-.en_US
dc.description.abstractNanoparticles are increasingly popular choices for labeling and tracking cells in biomedical applications such as cell therapy. However, all current types of nanoparticles fail to provide real-time, noninvasive monitoring of cell status and functions while often generating false positive signals. Herein, a nanosensor platform to track the real-time expression of specific biomarkers that correlate with cell status and functions is reported. Nanosensors are synthesized by encapsulating various sensor molecules within biodegradable polymeric nanoparticles. Upon intracellular entry, nanosensors reside within the cell cytoplasm, serving as a depot to continuously release sensor molecules for up to 30 days. In the absence of the target biomarkers, the released sensor molecules remain ‘Off’. When the biomarker(s) is expressed, a detectable signal is generated (On). As a proof-of-concept, three nanosensor formulations were synthesized to monitor cell viability, secretion of nitric oxide, and β-actin mRNA expression.en_US
dc.relation.ispartofseriesScientific Reportsen_US
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
dc.titleA Nanoparticle-based Sensor Platform for Cell Tracking and Status/Function Assessmenten_US
dc.typeJournal Article
dc.contributor.researchNTU-Northwestern Institute for Nanomedicineen_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.description.versionPublished versionen_US

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