dc.contributor.authorLai, Lan-Tian
dc.contributor.authorLee, Pin Jie
dc.contributor.authorZhang, Li-Feng
dc.identifier.citationLai, L.-T., Lee, P. J., & Zhang, L.-F. (2013). Immunofluorescence protects RNA signals in simultaneous RNA–DNA FISH. Experimental Cell Research, 319(3), 46-55.en_US
dc.description.abstractCell research often requires combinational detection of RNA and DNA by fluorescence in situ hybridization (RNA–DNA FISH). However, it is difficult to preserve the fragile RNA signals through the harsh conditions used to denature the DNA template in DNA FISH. The current protocols of RNA –DNA FISH still cannot work robustly in all experiments. RNA–DNA FISH remains as a technically challenging and tedious experiment. By incorporating protein components into the signal detection steps of RNA FISH, which is then followed by a post-fixation step, we established an improved protocol of RNA–DNA FISH. The established method worked satisfyingly and robustly in our studies on Xist (inactivated X chromosome specific transcript) RNA and Terra (telomeric repeat-containing RNA). Our results provided the direct evidence to show that, not all the telomeres are associated with Terra, and a significant fraction of Terra foci do not overlap with telomere DNA in interphase cell nuclei. The improved method of simultaneous RNA–DNA FISH is reliable and time-efficient. It can be used in a variety of biological studies.en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.format.extent22 p.en_US
dc.relation.ispartofseriesExperimental Cell Researchen_US
dc.rights© 2012 Elsevier Inc. All rights reserved. This is the author created version of a work that has been peer reviewed and accepted for publication by Experimental Cell Research, Elsevier Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.yexcr.2012.11.009]en_US
dc.subjectFluorescence in situ hybridization (FISH)en_US
dc.subjectSimultaneous RNA–DNA FISHen_US
dc.subjectX chromosome inactivation (XCI)en_US
dc.titleImmunofluorescence protects RNA signals in simultaneous RNA–DNA FISHen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionAccepted versionen_US

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