Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment
Author
Low, Wei Ching
Rujitanaroj, Pim-On
Lee, Dong-Keun
Kuang, Jinghao
Messersmith, Phillip B.
Chan, Jerry Kok Yen
Chew, Sing Yian
Date of Issue
2015School
School of Chemical and Biomedical Engineering
Lee Kong Chian School of Medicine (LKCMedicine)
Lee Kong Chian School of Medicine (LKCMedicine)
Version
Accepted version
Abstract
In this study, we promote neuronal differentiation of human mesenchymal stem cells (MSCs) through scaffold-mediated sustained release of siRNA targeting RE-1 silencing transcription factor (REST). Poly (ϵ-caprolactone) nanofibers were surface modified with mussel inspired DOPA-melanin (DM) coating for adsorption of REST siRNA. DM modification increased siRNA-loading efficiency and reduced the initial burst release. Fiber alignment and DM modification enhanced REST knockdown efficiencies. Under non-specific differentiation condition, REST silencing and fiber topography enhanced MSC neuronal markers expressions and reduced glial cell commitment. Such scaffolds may find useful applications in enhancing MSCs neuronal differentiation under non-specific conditions such as an in vivo environment.
Subject
Electrospinning
Neuronal differentiation
Neural differentiation
RE-1 silencing transcription factor
Sustained release
Neuronal differentiation
Neural differentiation
RE-1 silencing transcription factor
Sustained release
Type
Journal Article
Series/Journal Title
Macromolecular Bioscience
Rights
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Macromolecular Bioscience, WILEY-VCH Verlag GmbH & Co. KGaA. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/mabi.201500101].
Collections
http://dx.doi.org/10.1002/mabi.201500101
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