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|Title:||Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment||Authors:||Low, Wei Ching
Messersmith, Phillip B.
Chan, Jerry Kok Yen
Chew, Sing Yian
RE-1 silencing transcription factor
|Issue Date:||2015||Source:||Low, W. C., Rujitanaroj, P.-O., Lee, D.-K., Kuang, J., Messersmith, P. B., Chan, J. K. Y., et al. (2015). Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment. Macromolecular Bioscience, 15(10), 1457-1468.||Series/Report no.:||Macromolecular Bioscience||Abstract:||In this study, we promote neuronal differentiation of human mesenchymal stem cells (MSCs) through scaffold-mediated sustained release of siRNA targeting RE-1 silencing transcription factor (REST). Poly (ϵ-caprolactone) nanofibers were surface modified with mussel inspired DOPA-melanin (DM) coating for adsorption of REST siRNA. DM modification increased siRNA-loading efficiency and reduced the initial burst release. Fiber alignment and DM modification enhanced REST knockdown efficiencies. Under non-specific differentiation condition, REST silencing and fiber topography enhanced MSC neuronal markers expressions and reduced glial cell commitment. Such scaffolds may find useful applications in enhancing MSCs neuronal differentiation under non-specific conditions such as an in vivo environment.||URI:||https://hdl.handle.net/10356/80820
|ISSN:||1616-5187||DOI:||http://dx.doi.org/10.1002/mabi.201500101||Rights:||© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Macromolecular Bioscience, WILEY-VCH Verlag GmbH & Co. KGaA. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/mabi.201500101].||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||LKCMedicine Journal Articles|
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