dc.contributor.authorToh, Ying Xiu
dc.contributor.authorGan, Victor
dc.contributor.authorBalakrishnan, Thavamalar
dc.contributor.authorZuest, Roland
dc.contributor.authorPoidinger, Michael
dc.contributor.authorWilson, Solomonraj
dc.contributor.authorAppanna, Ramapraba
dc.contributor.authorThein, Tun Linn
dc.contributor.authorOng, Adrian Kheng-Yeow
dc.contributor.authorNg, Lee Ching
dc.contributor.authorLeo, Yee Sin
dc.contributor.authorFink, Katja
dc.identifier.citationToh, Y. X., Gan, V., Balakrishnan, T., Zuest, R., Poidinger, M., Wilson, S., et al. (2014). Dengue Serotype Cross-Reactive, Anti-E Protein Antibodies Confound Specific Immune Memory for 1 Year after Infection. Frontiers in Immunology, 5(388).en_US
dc.description.abstractDengue virus has four serotypes and is endemic globally in tropical countries. Neither a specific treatment nor an approved vaccine is available, and correlates of protection are not established. The standard neutralization assay cannot differentiate between serotype-specific and serotype cross-reactive antibodies in patients early after infection, leading to an overestimation of the long-term serotype-specific protection of an antibody response. It is known that the cross-reactive response in patients is temporary but few studies have assessed kinetics and potential changes in serum antibody specificity over time. To better define the specificity of polyclonal antibodies during disease and after recovery, longitudinal samples from patients with primary or secondary DENV-2 infection were collected over a period of 1 year. We found that serotype cross-reactive antibodies peaked 3 weeks after infection and subsided within 1 year. Since secondary patients rapidly produced antibodies specific for the virus envelope (E) protein, an E-specific ELISA was superior compared to a virus particle-specific ELISA to identify patients with secondary infections. Dengue infection triggered a massive activation and mobilization of both naïve and memory B cells possibly from lymphoid organs into the blood, providing an explanation for the surge of circulating plasmablasts and the increase in cross-reactive E protein-specific antibodies.en_US
dc.format.extent12 p.en_US
dc.relation.ispartofseriesFrontiers in Immunologyen_US
dc.rights© 2014 Toh, Gan, Balakrishnan, Zuest, Poidinger, Wilson, Appanna, Thein, Ong, Ng, Leo and Fink. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.subjectViral infectionen_US
dc.subjectB cellsen_US
dc.subjectLongitudinal studiesen_US
dc.titleDengue Serotype Cross-Reactive, Anti-E Protein Antibodies Confound Specific Immune Memory for 1 Year after Infectionen_US
dc.typeJournal Article
dc.contributor.schoolLee Kong Chian School of Medicine
dc.description.versionPublished versionen_US
dc.contributor.organizationLee Kong Chian School of Medicineen_US

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