View Item 
      •   Home
      • 1. Schools
      • College of Engineering
      • School of Materials Science and Engineering (MSE)
      • MSE Journal Articles
      • View Item
      •   Home
      • 1. Schools
      • College of Engineering
      • School of Materials Science and Engineering (MSE)
      • MSE Journal Articles
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.
      Subject Lookup

      Browse

      All of DR-NTUCommunities & CollectionsTitlesAuthorsBy DateSubjectsThis CollectionTitlesAuthorsBy DateSubjects

      My Account

      Login

      Statistics

      Most Popular ItemsStatistics by Country/RegionMost Popular Authors

      About DR-NTU

      Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels

      Thumbnail
      Smooth muscle cell alignment and phenotype control by melt spun polycaprolactone fibers for seeding of tissue engineered blood vessels.pdf (3.752Mb)
      Author
      Agrawal, Animesh
      Lee, Bae Hoon
      Irvine, Scott Alexander
      An, Jia
      Bhuthalingam, Ramya
      Singh, Vaishali
      Low, Kok Yao
      Chua, Chee Kai
      Venkatraman, Subbu Subramanian
      Date of Issue
      2015
      School
      School of Materials Science and Engineering
      School of Mechanical and Aerospace Engineering
      School of Biological Sciences
      Version
      Published version
      Abstract
      A method has been developed to induce and retain a contractile phenotype for vascular smooth muscle cells, as the first step towards the development of a biomimetic blood vessel construct with minimal compliance mismatch. Melt spun PCL fibers were deposited on a mandrel to form aligned fibers of 10 μm in diameter. The fibers were bonded into aligned arrangement through dip coating in chitosan solution. This formed a surface of parallel grooves, 10 μm deep by 10 μm across, presenting a surface layer of chitosan to promote cell surface interactions. The aligned fiber surface was used to culture cells present in the vascular wall, in particular fibroblasts and smooth muscle cells. This topography induced “surface guidance” over the orientation of the cells, which adopted an elongated spindle-like morphology, whereas cells on the unpatterned control surface did not show such orientation, assuming more rhomboid shapes. The preservation of VSMC contractile phenotype on the aligned scaffold was demonstrated by the retention of α-SMA expression after several days of culture. The effect was assessed on a prototype vascular graft prosthesis fabricated from polylactide caprolactone; VSMCs aligned longitudinally along a fiberless tube, whereas, for the aligned fiber coated tubes, the VSMCs aligned in the required circumferential orientation.
      Type
      Journal Article
      Series/Journal Title
      International Journal of Biomaterials
      Rights
      © 2015 Animesh Agrawal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
      Collections
      • MAE Journal Articles
      • MSE Journal Articles
      • SBS Journal Articles
      http://dx.doi.org/10.1155/2015/434876
      Get published version (via Digital Object Identifier)

      Show full item record


      NTU Library, Nanyang Avenue, Singapore 639798 © 2011 Nanyang Technological University. All rights reserved.
      DSpace software copyright © 2002-2015  DuraSpace
      Contact Us | Send Feedback
      Share |    
      Theme by 
      Atmire NV
       

       


      NTU Library, Nanyang Avenue, Singapore 639798 © 2011 Nanyang Technological University. All rights reserved.
      DSpace software copyright © 2002-2015  DuraSpace
      Contact Us | Send Feedback
      Share |    
      Theme by 
      Atmire NV
       

       

      DCSIMG