dc.contributor.authorAgrawal, Animesh
dc.contributor.authorLee, Bae Hoon
dc.contributor.authorIrvine, Scott Alexander
dc.contributor.authorAn, Jia
dc.contributor.authorBhuthalingam, Ramya
dc.contributor.authorSingh, Vaishali
dc.contributor.authorLow, Kok Yao
dc.contributor.authorChua, Chee Kai
dc.contributor.authorVenkatraman, Subbu Subramanian
dc.date.accessioned2015-12-14T02:49:16Z
dc.date.available2015-12-14T02:49:16Z
dc.date.issued2015
dc.identifier.citationAgrawal, A., Lee, B. H., Irvine, S. A., An, J., Bhuthalingam, R., Singh, V., et al. (2015). Smooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vessels. International Journal of Biomaterials, 2015, 434876-.en_US
dc.identifier.issn1687-8787en_US
dc.identifier.urihttp://hdl.handle.net/10220/39072
dc.description.abstractA method has been developed to induce and retain a contractile phenotype for vascular smooth muscle cells, as the first step towards the development of a biomimetic blood vessel construct with minimal compliance mismatch. Melt spun PCL fibers were deposited on a mandrel to form aligned fibers of 10 μm in diameter. The fibers were bonded into aligned arrangement through dip coating in chitosan solution. This formed a surface of parallel grooves, 10 μm deep by 10 μm across, presenting a surface layer of chitosan to promote cell surface interactions. The aligned fiber surface was used to culture cells present in the vascular wall, in particular fibroblasts and smooth muscle cells. This topography induced “surface guidance” over the orientation of the cells, which adopted an elongated spindle-like morphology, whereas cells on the unpatterned control surface did not show such orientation, assuming more rhomboid shapes. The preservation of VSMC contractile phenotype on the aligned scaffold was demonstrated by the retention of α-SMA expression after several days of culture. The effect was assessed on a prototype vascular graft prosthesis fabricated from polylactide caprolactone; VSMCs aligned longitudinally along a fiberless tube, whereas, for the aligned fiber coated tubes, the VSMCs aligned in the required circumferential orientation.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.format.extent8 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesInternational Journal of Biomaterialsen_US
dc.rights© 2015 Animesh Agrawal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.titleSmooth Muscle Cell Alignment and Phenotype Control by Melt Spun Polycaprolactone Fibers for Seeding of Tissue Engineered Blood Vesselsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1155/2015/434876
dc.description.versionPublished versionen_US


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