Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81115
Title: Functional VEGFA knockdown with artificial 3′-tailed mirtrons defined by 5′ splice site and branch point
Authors: Kock, Kian Hong
Kong, Kiat Whye
Hoon, Shawn
Seow, Yiqi
Keywords: Biological Sciences
Issue Date: 2015
Source: Kock, K. H., Kong, K. W., Hoon, S., & Seow, Y. (2015). Functional VEGFA knockdown with artificial 3′-tailed mirtrons defined by 5′ splice site and branch point. Nucleic Acids Research, 43(13), 6568-6578.
Series/Report no.: Nucleic Acids Research
Abstract: Mirtrons are introns that form pre-miRNA hairpins after splicing to produce RNA interference (RNAi) effectors distinct from Drosha-dependent intronic miRNAs, and will be especially useful for co-delivery of coding genes and RNAi. A specific family of mirtrons – 3′-tailed mirtrons – has hairpins precisely defined on the 5′ end by the 5′ splice site and 3′ end by the branch point. Here, we present design principles for artificial 3′-tailed mirtrons and demonstrate, for the first time, efficient gene knockdown with tailed mirtrons within eGFP coding region. These artificial tailed mirtrons, unlike canonical mirtrons, have very few sequence design restrictions. Tailed mirtrons targeted against VEGFA mRNA, the misregulation of which is causative of several disorders including cancer, achieved significant levels of gene knockdown. Tailed mirtron-mediated knockdown was further shown to be splicing-dependent, and at least as effective as equivalent artificial intronic miRNAs, with the added advantage of very defined cleavage sites for generation of mature miRNA guide strands. Further development and exploitation of this unique mirtron biogenesis pathway for therapeutic RNAi coupled into protein-expressing genes can potentially enable the development of precisely controlled combinatorial gene therapy.
URI: https://hdl.handle.net/10356/81115
http://hdl.handle.net/10220/39104
ISSN: 0305-1048
DOI: http://dx.doi.org/10.1093/nar/gkv617
Rights: © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
metadata.item.grantfulltext: open
metadata.item.fulltext: With Fulltext
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