dc.contributor.authorKock, Kian Hong
dc.contributor.authorKong, Kiat Whye
dc.contributor.authorHoon, Shawn
dc.contributor.authorSeow, Yiqi
dc.date.accessioned2015-12-16T08:49:30Z
dc.date.available2015-12-16T08:49:30Z
dc.date.issued2015
dc.identifier.citationKock, K. H., Kong, K. W., Hoon, S., & Seow, Y. (2015). Functional VEGFA knockdown with artificial 3′-tailed mirtrons defined by 5′ splice site and branch point. Nucleic Acids Research, 43(13), 6568-6578.en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10220/39104
dc.description.abstractMirtrons are introns that form pre-miRNA hairpins after splicing to produce RNA interference (RNAi) effectors distinct from Drosha-dependent intronic miRNAs, and will be especially useful for co-delivery of coding genes and RNAi. A specific family of mirtrons – 3′-tailed mirtrons – has hairpins precisely defined on the 5′ end by the 5′ splice site and 3′ end by the branch point. Here, we present design principles for artificial 3′-tailed mirtrons and demonstrate, for the first time, efficient gene knockdown with tailed mirtrons within eGFP coding region. These artificial tailed mirtrons, unlike canonical mirtrons, have very few sequence design restrictions. Tailed mirtrons targeted against VEGFA mRNA, the misregulation of which is causative of several disorders including cancer, achieved significant levels of gene knockdown. Tailed mirtron-mediated knockdown was further shown to be splicing-dependent, and at least as effective as equivalent artificial intronic miRNAs, with the added advantage of very defined cleavage sites for generation of mature miRNA guide strands. Further development and exploitation of this unique mirtron biogenesis pathway for therapeutic RNAi coupled into protein-expressing genes can potentially enable the development of precisely controlled combinatorial gene therapy.en_US
dc.format.extent11 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesNucleic Acids Researchen_US
dc.rights© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectBiological Sciences
dc.titleFunctional VEGFA knockdown with artificial 3′-tailed mirtrons defined by 5′ splice site and branch pointen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1093/nar/gkv617
dc.description.versionPublished versionen_US


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