Gold nanorods as photothermal agents and autofluorescence enhancer to track cell death during plasmonic photothermal therapy
Kannadorai, Ravi Kumar
Chiew, Geraldine Giap Ying
Luo, Kathy Qian
Date of Issue2015
European Conferences on Biomedical Optics
School of Chemical and Biomedical Engineering
The transverse and longitudinal plasmon resonance in gold nanorods can be exploited to localize the photothermal therapy and influence the fluorescence to monitor the treatment outcome at the same time. While the longitudinal plasmon peak contributes to the photothermal effect, the transverse peak can enhance fluorescence. After cells take in PEGylated nanorods through endocytosis, autofluorescence from endogenous fluorophores such as nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) in the mitochondria is enhanced two times, which is a good indicator of the respiratory status of the cell. When cells are illuminated continuously with near infrared laser, the temperature reaches the hyperthermic region within the first four minutes, which demonstrates the efficiency of gold nanorods in photothermal therapy. The cell viability test and autofluorescence intensity show good correlation indicating the progress of cell death over time.
Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV
© 2015 Society of Photo-optical Instrumentation Engineers. This paper was published in Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV and is made available as an electronic reprint (preprint) with permission of Society of Photo-optical Instrumentation Engineers. The published version is available at: [http://dx.doi.org/10.1117/12.2183581]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.