Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation
Author
Cao, Yuan
Nguyen, Kien Truc Giang
Tam, James P.
Liu, Chuan-Fa
Date of Issue
2015School
School of Biological Sciences
Version
Accepted version
Abstract
Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.
Subject
thioester
catalytic efficiency
catalytic efficiency
Type
Journal Article
Series/Journal Title
Chemical Communications
Rights
© 2015 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Chemical Communications, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/C5CC07227A].
Collections
http://dx.doi.org/10.1039/C5CC07227A
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