dc.contributor.authorCao, Yuan
dc.contributor.authorNguyen, Kien Truc Giang
dc.contributor.authorTam, James P.
dc.contributor.authorLiu, Chuan-Fa
dc.identifier.citationCao, Y., Nguyen, G. K. T., Tam, J. P., & Liu, C.-F. (2015). Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation. Chem. Commun., 51(97), 17289-17292.en_US
dc.description.abstractUsing a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.format.extent4 p.en_US
dc.relation.ispartofseriesChemical Communicationsen_US
dc.rights© 2015 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Chemical Communications, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/C5CC07227A].en_US
dc.subjectcatalytic efficiency
dc.titleButelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligationen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionAccepted versionen_US

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