dc.contributor.authorLiu, Xiaohu
dc.contributor.authorWang, Yi
dc.contributor.authorChen, Peng
dc.contributor.authorWang, Yusong
dc.contributor.authorZhang, Jinling
dc.contributor.authorAili, Daniel
dc.contributor.authorLiedberg, Bo
dc.date.accessioned2016-01-22T01:42:34Z
dc.date.available2016-01-22T01:42:34Z
dc.date.issued2014
dc.identifier.citationLiu, X., Wang, Y., Chen, P., Wang, Y., Zhang, J., Aili, D., et al. (2014). Biofunctionalized Gold Nanoparticles for Colorimetric Sensing of Botulinum Neurotoxin A Light Chain. Analytical Chemistry, 86(5), 2345-2352.en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://hdl.handle.net/10220/39747
dc.description.abstractBotulinum neurotoxin is considered as one of the most toxic food-borne substances and is a potential bioweapon accessible to terrorists. The development of an accurate, convenient, and rapid assay for botulinum neurotoxins is therefore highly desirable for addressing biosafety concerns. Herein, novel biotinylated peptide substrates designed to mimic synaptosomal-associated protein 25 (SNAP-25) are utilized in gold nanoparticle-based assays for colorimetric detection of botulinum neurotoxin serotype A light chain (BoLcA). In these proteolytic assays, biotinylated peptides serve as triggers for the aggregation of gold nanoparticles, while the cleavage of these peptides by BoLcA prevents nanoparticle aggregation. Two different assay strategies are described, demonstrating limits of detection ranging from 5 to 0.1 nM of BoLcA with an overall assay time of 4 h. These hybrid enzyme-responsive nanomaterials provide rapid and sensitive detection for one of the most toxic substances known to man.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.format.extent24 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesAnalytical Chemistryen_US
dc.rights© 2014 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical Chemistry, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/ac402626g].en_US
dc.subjectBiological weapons
dc.subjectAssays
dc.titleBiofunctionalized Gold Nanoparticles for Colorimetric Sensing of Botulinum Neurotoxin A Light Chainen_US
dc.typeJournal Article
dc.contributor.researchCentre for Biomimetic Sensor Scienceen_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.identifier.doihttp://dx.doi.org/10.1021/ac402626g
dc.description.versionAccepted versionen_US
dc.contributor.organizationCentre for Biomimetic Sensor Scienceen_US


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