dc.contributor.authorYang, Renliang
dc.contributor.authorBi, Xiaobao
dc.contributor.authorLi, Fupeng
dc.contributor.authorCao, Yuan
dc.contributor.authorLiu, Chuan-Fa
dc.date.accessioned2016-01-22T03:58:42Z
dc.date.available2016-01-22T03:58:42Z
dc.date.issued2014
dc.identifier.citationYang, R., Bi, X., Li, F., Cao, Y., & Liu, C.-F. (2014). Native chemical ubiquitination using a genetically incorporated azidonorleucine. Chemical Communications, 50(59), 7971-7974.en_US
dc.identifier.issn1359-7345en_US
dc.identifier.urihttp://hdl.handle.net/10220/39753
dc.description.abstractA robust chemical ubiquitination method was developed. The method employed a genetically incorporated azidonorleucine as an orthogonal lysine precursor for the installation of a Gly residue bearing an Nα-auxiliary which mediated the ligation between ubiquitin(1–75)-thioester and the target protein. To demonstrate our methodology, a model protein, K48-linked diubiquitin, was synthesized with an overall yield of 35%.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.format.extent4 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesChemical Communicationsen_US
dc.rights© 2014 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Chemical Communications, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/c4cc03721a].en_US
dc.subjectBiological Sciences
dc.titleNative chemical ubiquitination using a genetically incorporated azidonorleucineen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1039/c4cc03721a
dc.description.versionAccepted versionen_US


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