dc.contributor.authorGuo, Wei Mei
dc.contributor.authorLoh, Xian Jun
dc.contributor.authorTan, Ern Yu
dc.contributor.authorLoo, Joachim Say Chye
dc.contributor.authorHo, Vincent H. B.
dc.identifier.citationGuo, W. M., Loh, X. J., Tan, E. Y., Loo, J. S. C., & Ho, V. H. B. (2014). Development of a Magnetic 3D Spheroid Platform with Potential Application for High-Throughput Drug Screening. Molecular Pharmaceutics, 11(7), 2182-2189.en_US
dc.description.abstractThree-dimensional (3D) cell culture has become increasingly adopted as a more accurate model of the complex in vivo microenvironment compared to conventional two-dimensional (2D) cell culture. Multicellular spheroids are important 3D cell culture models widely used in biological studies and drug screening. To facilitate simple spheroid manipulation, magnetic spheroids were generated from magnetically labeled cells using a scaffold-free approach. This method is applicable to a variety of cell types. The spheroids generated can be targeted and immobilized using magnetic field gradients, allowing media change or dilution to be performed with minimal disruption to the spheroids. Cells in magnetic spheroids showed good viability and displayed typical 3D morphology. Using this platform, a 28 day study was carried out using doxorubicin on magnetic MCF-7 spheroids. The results provided a proof-of-principle for using magnetic tumor spheroids in therapeutic studies. They can offer beneficial insights that help to bridge the gap between in vitro and in vivo models. Furthermore, this platform can be adapted for high-throughput screening in drug discovery.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.description.sponsorshipMOH (Min. of Health, S’pore)en_US
dc.format.extent23 p.en_US
dc.relation.ispartofseriesMolecular Pharmaceuticsen_US
dc.rights© 2014 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Molecular Pharmaceutics, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/mp5000604].en_US
dc.subjectmulticellular spheroids; magnetic cell labeling; spheroid manipulation; therapeutic studies; high-throughput screeningen_US
dc.titleDevelopment of a Magnetic 3D Spheroid Platform with Potential Application for High-Throughput Drug Screeningen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.description.versionAccepted versionen_US

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