dc.contributor.authorFoo, Jee Loon
dc.contributor.authorJensen, Heather M.
dc.contributor.authorDahl, Robert H.
dc.contributor.authorGeorge, Kevin
dc.contributor.authorKeasling, Jay D.
dc.contributor.authorLee, Taek Soon
dc.contributor.authorLeong, Susanna
dc.contributor.authorMukhopadhyay, Aindrila
dc.identifier.citationFoo, J. L., Jensen, H. M., Dahl, R. H., George, K., Keasling, J. D., Lee, T. S., et al. (2014). Improving Microbial Biogasoline Production in Escherichia coli Using Tolerance Engineering. mBio, 5(6), e01932-14.en_US
dc.description.abstractEngineering microbial hosts for the production of fungible fuels requires mitigation of limitations posed on the production capacity. One such limitation arises from the inherent toxicity of solvent-like biofuel compounds to production strains, such as Escherichia coli. Here we show the importance of host engineering for the production of short-chain alcohols by studying the overexpression of genes upregulated in response to exogenous isopentenol. Using systems biology data, we selected 40 genes that were upregulated following isopentenol exposure and subsequently overexpressed them in E. coli. Overexpression of several of these candidates improved tolerance to exogenously added isopentenol. Genes conferring isopentenol tolerance phenotypes belonged to diverse functional groups, such as oxidative stress response (soxS, fpr, and nrdH), general stress response (metR, yqhD, and gidB), heat shock-related response (ibpA), and transport (mdlB). To determine if these genes could also improve isopentenol production, we coexpressed the tolerance-enhancing genes individually with an isopentenol production pathway. Our data show that expression of 6 of the 8 candidates improved the production of isopentenol in E. coli, with the methionine biosynthesis regulator MetR improving the titer for isopentenol production by 55%. Additionally, expression of MdlB, an ABC transporter, facilitated a 12% improvement in isopentenol production. To our knowledge, MdlB is the first example of a transporter that can be used to improve production of a short-chain alcohol and provides a valuable new avenue for host engineering in biogasoline production.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.format.extent9 p.en_US
dc.rights© 2014 Foo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.titleImproving Microbial Biogasoline Production in Escherichia coli Using Tolerance Engineeringen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.description.versionPublished versionen_US

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