dc.contributor.authorXiao, Keke
dc.contributor.authorGuo, Chenghong
dc.contributor.authorZhou, Yan
dc.contributor.authorMaspolim, Yogananda
dc.contributor.authorNg, Wun-Jern
dc.date.accessioned2016-03-15T07:23:05Z
dc.date.available2016-03-15T07:23:05Z
dc.date.issued2015
dc.identifier.citationXiao, K., Guo, C., Zhou, Y., Maspolim, Y., & Ng, W.-J. (2016). Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system. Chemosphere, 144, 1498-1504.en_US
dc.identifier.issn0045-6535en_US
dc.identifier.urihttp://hdl.handle.net/10220/40285
dc.description.abstractThis study reports on biomass tolerance towards high concentrations of acetic acid (HAc) within the system. Biomass from the second stage of a two-stage anaerobic sludge digestion system was used for this study. Microbial community analysis by 454 pyrosequencing highlighted hydrogenotrophic Methanomicrobiales was the predominant archaeal population in the second stage (>99% of the total archaeal community). Second stage biomass degraded HAc up to 4200 mg HAc L-1 without observable lag phase. However, at HAc-shock loading of 7400 mg HAc L-1, it showed a one day lag phase associated with decreased biomass activity. After stepwise HAc-acclimation over 27 d, the biomass degraded HAc of up to 8200 mg HAc L-1 without observable lag phase. The dominance of Methanomicrobiales had remained unchanged in proportion - while the total archaeal population increased during acclimation. This study showed stepwise acclimation could be an approach to accommodate HAc accumulation and hence higher concentrations resulting from an enhanced first stage. Keywords: Acclimation; Acetic acid inhibition; Biomass activity; Hydrogenotrophic methanogens; Pyrosequencing; Two-stage anaerobic systemen_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.format.extent20 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesChemosphereen_US
dc.rights© 2015 Elsevier Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Chemosphere, Elsevier Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.chemosphere.2015.10.035].en_US
dc.subjectTwo-stage anaerobic systemen_US
dc.subjectAcetic acid inhibitionen_US
dc.subjectAcclimationen_US
dc.subjectHydrogenotrophic methanogensen_US
dc.subjectPyrosequencingen_US
dc.subjectBiomass activityen_US
dc.titleAcetic acid effects on methanogens in the second stage of a two-stage anaerobic systemen_US
dc.typeJournal Article
dc.contributor.researchNanyang Environment and Water Research Instituteen_US
dc.contributor.schoolSchool of Civil and Environmental Engineeringen_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.chemosphere.2015.10.035
dc.description.versionAccepted versionen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record