dc.contributor.authorSerra, Aida
dc.contributor.authorZhu, Hongbin
dc.contributor.authorGallart-Palau, Xavier
dc.contributor.authorPark, Jung Eun
dc.contributor.authorHo, Hee Haw
dc.contributor.authorTam, James P.
dc.contributor.authorSze, Siu Kwan
dc.date.accessioned2016-04-15T06:33:16Z
dc.date.available2016-04-15T06:33:16Z
dc.date.issued2016
dc.identifier.citationSerra, A., Zhu, H., Gallart-Palau, X., Park, J. E., Ho, H. H., Tam, J. P., et al. (2016). Plasma proteome coverage is increased by unique peptide recovery from sodium deoxycholate precipitate. Analytical and Bioanalytical Chemistry, 408(7), 1963-1973.en_US
dc.identifier.issn1618-2642en_US
dc.identifier.urihttp://hdl.handle.net/10220/40445
dc.description.abstractThe ionic detergent sodium deoxycholate (SDC) is compatible with in-solution tryptic digestion and LC-MS/MS-based shotgun proteomics by virtue of being easy to separate from the peptide products via precipitation in acidic buffers. However, it remains unclear whether unique human peptides co-precipitate with SDC during acid treatment of complex biological samples. In this study, we demonstrate for the first time that a large quantity of unique peptides in human blood plasma can be co-precipitated with SDC using an optimized sample preparation method prior to shotgun proteomic analysis. We show that the plasma peptides co-precipitated with SDC can be successfully recovered using a sequential re-solubilization and precipitation procedure, and that this approach is particularly efficient at the extraction of long peptides. Recovery of peptides from the SDC pellet dramatically increased overall proteome coverage (>60 %), thereby improving the identification of low-abundance proteins and enhancing the identification of protein components of membrane-bound organelles. In addition, when we analyzed the physiochemical properties of the co-precipitated peptides, we observed that SDC-based sample preparation improved the identification of mildly hydrophilic/hydrophobic proteins that would otherwise be lost upon discarding the pellet. These data demonstrate that the optimized SDC protocol is superior to sodium dodecyl sulfate (SDS)/urea treatment for identifying plasma biomarkers by shotgun proteomics.en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent31 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesAnalytical and Bioanalytical Chemistryen_US
dc.rights© 2016 Springer Berlin Heidelberg. This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical and Bioanalytical Chemistry, Springer Berlin Heidelberg. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s00216-016-9312-7].en_US
dc.subjectPlasma proteomeen_US
dc.subjectMass spectrometryen_US
dc.subjectShotgun proteomicsen_US
dc.subjectSodium deoxycholateen_US
dc.subjectTrypsin digestionen_US
dc.titlePlasma proteome coverage is increased by unique peptide recovery from sodium deoxycholate precipitateen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1007/s00216-016-9312-7
dc.description.versionAccepted versionen_US


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