Rupture of zwitterionic lipid vesicles by an amphipathic, α-helical peptide: Indirect effects of sensor surface and implications for experimental analysis
Author
Zan, Goh Haw
Cho, Nam-Joon
Date of Issue
2014School
School of Chemical and Biomedical Engineering
School of Materials Science and Engineering
School of Materials Science and Engineering
Research Centre
Centre for Biomimetic Sensor Science
Abstract
Surface-based measurement approaches offer competitive advantages for studying lipid membranes and membrane-active drug candidates. Continued development of surface-based membrane assays for pharmaceutical and biotechnology applications should account for physicochemical properties of the sensor surface. As a model system, the rupture of surface-adsorbed, zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid vesicles by an amphipathic, α-helical (AH) peptide, which attaches to the vesicle surface and destabilizes the membrane, was investigated herein. The kinetics of vesicle rupture on three different sensor surfaces (gold, titanium oxide, and aluminum oxide) were tracked by simultaneous quartz crystal microbalance-dissipation (QCM-D) and ellipsometry. On all three surfaces, complete vesicle rupture was observed. However, the corresponding kinetics of the vesicle rupture process and its sub-steps varied significantly between the three surfaces. In particular, the experimental data supports that the vesicle–substrate interaction influences the rupture process via differences in vesicle shape and lipid–substrate adhesion.
Subject
Quartz crystal microbalance-dissipation (QCM-D)
Ellipsometry
Vesicle
Peptide
Lipid bilayer
Ellipsometry
Vesicle
Peptide
Lipid bilayer
Type
Journal Article
Series/Journal Title
Colloids and Surfaces B: Biointerfaces
Rights
© 2014 Elsevier
Collections
http://dx.doi.org/10.1016/j.colsurfb.2014.06.014
Get published version (via Digital Object Identifier)