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      Rupture of zwitterionic lipid vesicles by an amphipathic, α-helical peptide: Indirect effects of sensor surface and implications for experimental analysis

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      Author
      Zan, Goh Haw
      Cho, Nam-Joon
      Date of Issue
      2014
      School
      School of Chemical and Biomedical Engineering
      School of Materials Science and Engineering
      Research Centre
      Centre for Biomimetic Sensor Science
      Abstract
      Surface-based measurement approaches offer competitive advantages for studying lipid membranes and membrane-active drug candidates. Continued development of surface-based membrane assays for pharmaceutical and biotechnology applications should account for physicochemical properties of the sensor surface. As a model system, the rupture of surface-adsorbed, zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid vesicles by an amphipathic, α-helical (AH) peptide, which attaches to the vesicle surface and destabilizes the membrane, was investigated herein. The kinetics of vesicle rupture on three different sensor surfaces (gold, titanium oxide, and aluminum oxide) were tracked by simultaneous quartz crystal microbalance-dissipation (QCM-D) and ellipsometry. On all three surfaces, complete vesicle rupture was observed. However, the corresponding kinetics of the vesicle rupture process and its sub-steps varied significantly between the three surfaces. In particular, the experimental data supports that the vesicle–substrate interaction influences the rupture process via differences in vesicle shape and lipid–substrate adhesion.
      Subject
      Quartz crystal microbalance-dissipation (QCM-D)
      Ellipsometry
      Vesicle
      Peptide
      Lipid bilayer
      Type
      Journal Article
      Series/Journal Title
      Colloids and Surfaces B: Biointerfaces
      Rights
      © 2014 Elsevier
      Collections
      • MSE Journal Articles
      • SCBE Journal Articles
      http://dx.doi.org/10.1016/j.colsurfb.2014.06.014
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