Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide
Author
Tabaei, Seyed Ruhollah
Cho, Nam Joon
Date of Issue
2015School
School of Chemical and Biomedical Engineering
School of Materials Science and Engineering
School of Materials Science and Engineering
Related Organization
Centre for Biomimetic Sensor Science
Abstract
Using total internal fluorescence microscopy, highly parallel measurements of single lipid vesicles unexpectedly reveal that a small fraction of vesicles rupture in multiple discrete steps when destabilized by a membrane-active peptide which is in contrast to classical solubilization models. To account for this surprizing kinetic behaviour, we identified that this vesicle subpopulation consists of multilamellar vesicles, and that the outermost lamella is more susceptible to rupture than unilamellar vesicles of even smaller size. This finding sheds light on the multiple ways in which membrane configuration can influence strain in the bilayer leaflet and contribute to nm-scale membrane curvature sensing.
Subject
Nanostructures
Type
Journal Article
Series/Journal Title
Chemical Communications
Rights
© 2015 The Royal Society of Chemistry.
Collections
http://dx.doi.org/10.1039/C5CC02769A
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