dc.contributor.authorOh, Eunkyul
dc.contributor.authorJackman, Joshua Alexander
dc.contributor.authorYorulmaz, Saziye
dc.contributor.authorZhdanov, Vladimir P.
dc.contributor.authorLee, Haiwon
dc.contributor.authorCho, Nam-Joon
dc.identifier.citationOh, E., Jackman, J. A., Yorulmaz, S., Zhdanov, V. P., Lee, H., & Cho, N.-J. (2015). Contribution of Temperature to Deformation of Adsorbed Vesicles Studied by Nanoplasmonic Biosensing. Langmuir, 31(2), 771-781.en_US
dc.description.abstractWith increasing temperature, biological macromolecules and nanometer-sized aggregates typically undergo complex and poorly understood reconfigurations, especially in the adsorbed state. Herein, we demonstrate the strong potential of using localized surface plasmon resonance (LSPR) sensors to address challenging questions related to this topic. By employing an LSPR-based gold nanodisk array platform, we have studied the adsorption of sub-100-nm diameter 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid vesicles on titanium oxide at two temperatures, 23 and 50 °C. Inside this temperature range, DPPC lipid vesicles undergo the gel-to-fluid phase transition accompanied by membrane area expansion, while DOPC lipid vesicles remain in the fluid-phase state. To interpret the corresponding measurement results, we have derived general equations describing the effect of deformation of adsorbed vesicles on the LSPR signal. At the two temperatures, the shape of adsorbed DPPC lipid vesicles on titanium oxide remains nearly equivalent, while DOPC lipid vesicles become less deformed at higher temperature. Adsorption and rupture of DPPC lipid vesicles on silicon oxide were also studied for comparison. In contrast to the results obtained on titanium oxide, adsorbed vesicles on silicon oxide become more deformed at higher temperature. Collectively, the findings demonstrate that increasing temperature may ultimately promote, hinder, or have negligible effect on the deformation of adsorbed vesicles. The physics behind these observations is discussed, and helps to clarify the interplay of various, often hidden, factors involved in adsorption of biological macromolecules at interfaces.en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.rights© 2014 American Chemical Society.en_US
dc.subjectChemical and Biomedical Engineeringen_US
dc.subjectMaterials Science and Engineeringen_US
dc.titleContribution of Temperature to Deformation of Adsorbed Vesicles Studied by Nanoplasmonic Biosensingen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US

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