Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/81061
Title: Silk fibroin–keratin based 3D scaffolds as a dermal substitute for skin tissue engineering
Authors: Bhardwaj, Nandana
Sow, Wan Ting
Devi, Dipali
Ng, Kee Woei
Mandal, Biman B.
Cho, Nam-Joon
Keywords: Chemical and Biomedical Engineering
Materials Science and Engineering
Issue Date: 2015
Source: Bhardwaj, N., Sow, W. T., Devi, D., Ng, K. W., Mandal, B. B., & Cho, N.-J. (2015). Silk fibroin–keratin based 3D scaffolds as a dermal substitute for skin tissue engineering. Integrative Biology, 7(1), 53-63.
Series/Report no.: Integrative Biology
Abstract: Development of highly vascular dermal tissue-engineered skin substitutes with appropriate mechanical properties and cellular cues is in need for significant advancement in the field of dermal reconstruction. Limitations have been imposed on natural biomaterials despite their superb biocompatibility hence, studies in biomaterial blending have been ongoing. Herein, we investigated blends of silk fibroin and human hair-derived keratin as wound-healing substrates that promote enhanced fibroblast cell adhesion and proliferation. Three-dimensional (3D) blended scaffolds were fabricated by freeze-drying, and their physico-chemical, mechanical and degradable properties were extensively characterized. Cytocompatibility tests observing cell adhesion and cell proliferation have shown significant enhancements in blended scaffolds. Also, its structural composition with high porosity (>85%) and interconnected pores in the range of 100–120 microns further confirms the superiority of the complex compared to its counterparts. FTIR studies identified the enhanced stability within its structure and were followed-up with sequential experiments to demonstrate improved thermal, degradation, and mechanical properties. Furthermore, immunohistochemical staining revealed greater expression of collagen type I in the cultured cells, indicating functional fibroblast proliferation and, hence, the exciting potential of this construct for dermal applications. Taken together, this study demonstrates the promising attributes from blended biomaterials and specifically present silk fibroin and human hair keratin blended scaffolds as a promising dermal substitute for skin tissue engineering.
URI: https://hdl.handle.net/10356/81061
http://hdl.handle.net/10220/40651
ISSN: 1757-9694
DOI: 10.1039/C4IB00208C
Rights: © 2015 Royal Society of Chemistry
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles
SCBE Journal Articles

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.