dc.contributor.authorBhardwaj, Nandana
dc.contributor.authorSow, Wan Ting
dc.contributor.authorDevi, Dipali
dc.contributor.authorNg, Kee Woei
dc.contributor.authorMandal, Biman B.
dc.contributor.authorCho, Nam-Joon
dc.date.accessioned2016-06-09T05:20:10Z
dc.date.available2016-06-09T05:20:10Z
dc.date.issued2015
dc.identifier.citationBhardwaj, N., Sow, W. T., Devi, D., Ng, K. W., Mandal, B. B., & Cho, N.-J. (2015). Silk fibroin–keratin based 3D scaffolds as a dermal substitute for skin tissue engineering. Integrative Biology, 7(1), 53-63.en_US
dc.identifier.issn1757-9694en_US
dc.identifier.urihttp://hdl.handle.net/10220/40651
dc.description.abstractDevelopment of highly vascular dermal tissue-engineered skin substitutes with appropriate mechanical properties and cellular cues is in need for significant advancement in the field of dermal reconstruction. Limitations have been imposed on natural biomaterials despite their superb biocompatibility hence, studies in biomaterial blending have been ongoing. Herein, we investigated blends of silk fibroin and human hair-derived keratin as wound-healing substrates that promote enhanced fibroblast cell adhesion and proliferation. Three-dimensional (3D) blended scaffolds were fabricated by freeze-drying, and their physico-chemical, mechanical and degradable properties were extensively characterized. Cytocompatibility tests observing cell adhesion and cell proliferation have shown significant enhancements in blended scaffolds. Also, its structural composition with high porosity (>85%) and interconnected pores in the range of 100–120 microns further confirms the superiority of the complex compared to its counterparts. FTIR studies identified the enhanced stability within its structure and were followed-up with sequential experiments to demonstrate improved thermal, degradation, and mechanical properties. Furthermore, immunohistochemical staining revealed greater expression of collagen type I in the cultured cells, indicating functional fibroblast proliferation and, hence, the exciting potential of this construct for dermal applications. Taken together, this study demonstrates the promising attributes from blended biomaterials and specifically present silk fibroin and human hair keratin blended scaffolds as a promising dermal substitute for skin tissue engineering.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.language.isoenen_US
dc.relation.ispartofseriesIntegrative Biologyen_US
dc.rights© 2015 Royal Society of Chemistryen_US
dc.subjectChemical and Biomedical Engineeringen_US
dc.subjectMaterials Science and Engineeringen_US
dc.titleSilk fibroin–keratin based 3D scaffolds as a dermal substitute for skin tissue engineeringen_US
dc.typeJournal Article
dc.contributor.researchCentre for Biomimetic Sensor Scienceen_US
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.identifier.doihttp://dx.doi.org/10.1039/C4IB00208C


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