dc.contributor.authorHo, Steven C. L.
dc.contributor.authorYang, Yuansheng
dc.identifier.citationHo, S. C. L., & Yang, Y. (2014). Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells. Biotechnology Letters, 36(8), 1569-1579.en_US
dc.description.abstractPromoters are essential on plasmid vectors to initiate transcription of the transgenes when generating therapeutic recombinant proteins expressing mammalian cell lines. High and sustained levels of gene expression are desired during therapeutic protein production while gene expression is useful for cell engineering. As many finely controlled promoters exhibit cell and product specificity, new promoters need to be identified, optimized and carefully evaluated before use. Suitable promoters can be identified using techniques ranging from simple molecular biology methods to modern high-throughput omics screenings. Promoter engineering is often required after identification to either obtain high and sustained expression or to provide a wider range of gene expression. This review discusses some of the available methods to identify and engineer promoters for therapeutic recombinant protein expression in mammalian cells.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.format.extent65 p.en_US
dc.relation.ispartofseriesBiotechnology Lettersen_US
dc.rights© 2014 Springer. This is the author created version of a work that has been peer reviewed and accepted for publication by Biotechnology Letters, Springer. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s10529-014-1523-4].en_US
dc.subjectMammalian cellsen_US
dc.subjectPromoter engineeringen_US
dc.subjectPromoter identificationen_US
dc.subjectRecombinant protein productionen_US
dc.titleIdentifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cellsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.description.versionAccepted versionen_US

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