dc.contributor.authorCarlevaro-Fita, Joana
dc.contributor.authorRahim, Anisa
dc.contributor.authorGuigó, Roderic
dc.contributor.authorVardy, Leah Karen Anne
dc.contributor.authorJohnson, Rory
dc.identifier.citationCarlevaro-Fita, J., Rahim, A., Guigó, R., Vardy, L. A., & Johnson, R. (2016). Cytoplasmic long noncoding RNAs are frequently bound to and degraded at ribosomes in human cells. RNA, 22(6), 867-882.en_US
dc.description.abstractRecent footprinting studies have made the surprising observation that long noncoding RNAs (lncRNAs) physically interact with ribosomes. However, these findings remain controversial, and the overall proportion of cytoplasmic lncRNAs involved is unknown. Here we make a global, absolute estimate of the cytoplasmic and ribosome-associated population of stringently filtered lncRNAs in a human cell line using polysome profiling coupled to spike-in normalized microarray analysis. Fifty-four percent of expressed lncRNAs are detected in the cytoplasm. The majority of these (70%) have >50% of their cytoplasmic copies associated with polysomal fractions. These interactions are lost upon disruption of ribosomes by puromycin. Polysomal lncRNAs are distinguished by a number of 5′ mRNA-like features, including capping and 5′UTR length. On the other hand, nonpolysomal “free cytoplasmic” lncRNAs have more conserved promoters and a wider range of expression across cell types. Exons of polysomal lncRNAs are depleted of endogenous retroviral insertions, suggesting a role for repetitive elements in lncRNA localization. Finally, we show that blocking of ribosomal elongation results in stabilization of many associated lncRNAs. Together these findings suggest that the ribosome is the default destination for the majority of cytoplasmic long noncoding RNAs and may play a role in their degradation.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.format.extent16 p.en_US
dc.rights© 2016 Carlevaro-Fita et al. This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.en_US
dc.subjectLong noncoding RNAen_US
dc.subjectTransposable elementen_US
dc.subjectRibosome profilingen_US
dc.titleCytoplasmic long noncoding RNAs are frequently bound to and degraded at ribosomes in human cellsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US

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