Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/93138
Title: Nanofiber-mediated microRNA delivery to enhance differentiation and maturation of oligodendroglial precursor cells
Authors: Diao, Hua Jia
Low, Wei Ching
Milbreta, Ulla
Lu, Q. Richard
Chew, Sing Yian
Keywords: Oligodendroglial precursor cells
Remyelination
Issue Date: 2015
Source: Diao, H. J., Low, W. C., Milbreta, U., Lu, Q. R., & Chew, S. Y. (2015). Nanofiber-mediated microRNA delivery to enhance differentiation and maturation of oligodendroglial precursor cells. Journal of Controlled Release, 208, 85-92.
Series/Report no.: Journal of Controlled Release
Abstract: Remyelination in the central nervous system (CNS) is critical in the treatment of many neural pathological conditions. Unfortunately, the ability to direct and enhance oligodendrocyte (OL) differentiation and maturation remains limited. It is known that microenvironmental signals, such as substrate topography and biochemical signaling, regulate cell fate commitment. Therefore, in this study, we developed a nanofiber-mediated microRNA (miR) delivery method to control oligodendroglial precursor cell (OPC) differentiation through a combination of fiber topography and gene silencing. Using poly(ε-caprolactone) nanofibers, efficient knockdown of OL differentiation inhibitory regulators were achieved by either nanofiber alone (20-40%, p<0.05) or the synergistic integration with miR-219 and miR-338 (up to 60%, p<0.05). As compared to two-dimensional culture, nanofiber topography enhanced OPC differentiation by inducing 2-fold increase in RIP(+) cells (p<0.01) while the presence of miRs further enhanced the result to 3-fold (p<0.001). In addition, nanofiber-mediated delivery of miR-219 and miR-338 promoted OL maturation by increasing the number of MBP(+) cells significantly (p<0.01). Taken together, the results demonstrate the efficacy of nanofibers in providing topographical cues and microRNA reverse transfection to direct OPC differentiation. Such scaffolds may find useful applications in directing oligodendrocyte differentiation and myelination for treatment of CNS pathological conditions that require remyelination.
URI: https://hdl.handle.net/10356/93138
http://hdl.handle.net/10220/40991
ISSN: 0168-3659
DOI: 10.1016/j.jconrel.2015.03.005
Schools: School of Chemical and Biomedical Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
Rights: © 2015 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Controlled Release, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.jconrel.2015.03.005].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SCBE Journal Articles

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