Antifungal properties of lecithin- and terbinafine-loaded electrospun poly(ε-caprolactone) nanofibres
Fazil, Mobashar Hussain Urf Turabe
Goh, Eunice Tze Leng
Ong, Seow Theng
Barathi, Veluchamy Amutha
Beuerman, Roger W.
Verma, Navin Kumar
Date of Issue2016
Lee Kong Chian School of Medicine
Topical skin and nail fungal infections form the most numerous and widespread among superficial mycoses. Drug eluting electrospun nanofibres have been shown to have immense potential for the topical delivery of antimicrobials. In this article, we investigated the efficacy of lecithin-loaded electrospun polycaprolactone (PCL) fibers containing terbinafine hydrochloride (terbinafine) for applications in superficial mycoses. Electron microscopy studies indicated that addition of lecithin and terbinafine decreased the average diameter of PCL nanofibers, increase in mechanical properties and wettability of the fibre mats. PCL mats containing lecithin and terbinafine displayed pronounced blue photoluminescence and did not affect cell adhesion and biocompatibility for primary human dermal fibroblasts. The drug loaded mats maintained the antifungal efficacy against moulds as well as dermatophytic fungus. Using an ex vivo porcine skin infection model, we showed that the drug-eluting mats resulted in >5 log reduction in the viability of T. mentagrophytes.
© 2016 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by RSC Advances, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/C6RA04755F].