dc.contributor.authorFox, Stephen John
dc.contributor.authorFazil, Mobashar Hussain Urf Turabe
dc.contributor.authorDhand, Chetna
dc.contributor.authorVenkatesh, Mayandi
dc.contributor.authorGoh, Eunice Tze Leng
dc.contributor.authorHarini, Sriram
dc.contributor.authorEugene, Christo
dc.contributor.authorLim, Rayne Rui
dc.contributor.authorRamakrishna, Seeram
dc.contributor.authorChaurasia, Shyam Sunder
dc.contributor.authorBeuerman, Roger W.
dc.contributor.authorVerma, Chandra Shekhar
dc.contributor.authorVerma, Navin Kumar
dc.contributor.authorLoh, Xian Jun
dc.contributor.authorLakshminarayanan, Rajamani
dc.identifier.citationFox, S. J., Fazil, M. H. U. T., Dhand, C., Venkatesh, M., Goh, E. T. L., Harini, S., et al. (2016). Insight into membrane selectivity of linear and branched polyethylenimines and their potential as biocides for advanced wound dressings. Acta Biomaterialia, 37, 155-164.en_US
dc.description.abstractWe report here structure-property relationship between linear and branched polyethylene imines by examining their antimicrobial activities against wide range of pathogens. Both the polymers target the cytoplasmic membrane of bacteria and yeasts, eliciting rapid microbicidal properties. Using multiscale molecular dynamic simulations, we showed that, in both fully or partially protonated forms LPEI discriminates between mammalian and bacterial model membranes whereas BPEI lacks selectivity for both the model membranes. Simulation results suggest that LPEI forms weak complex with the zwitterionic lipids whereas the side chain amino groups of BPEI sequester the zwitterionic lipids by forming tight complex. Consistent with these observations, label-free cell impedance measurements, cell viability assays and high content analysis indicate that BPEI is cytotoxic to human epithelial and fibroblasts cells. Crosslinking of BPEI onto electrospun gelatin mats attenuate the cytotoxicity for fibroblasts while retaining the antimicrobial activity against Gram-positive and yeasts strains. PEI crosslinked gelatin mats elicit bactericidal activity by contact-mediated killing and durable to leaching for 7days. The potent antimicrobial activity combined with enhanced selectivity of the crosslinked ES gelatin mats would expand the arsenel of biocides in the management of superficial skin infections. The contact-mediated microbicidal properties may avert antimicrobial resistance and expand the diversity of applications to prevent microbial contamination.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.format.extent55 p.en_US
dc.relation.ispartofseriesActa Biomaterialiaen_US
dc.rights© 2016 Acta Materialia Inc. (published by Elsevier Ltd.). This is the author created version of a work that has been peer reviewed and accepted for publication in Acta Biomaterialia, published by Elsevier Ltd. on behalf of Acta Materialia Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document.  The published version is available at: [http://dx.doi.org/10.1016/j.actbio.2016.04.015].en_US
dc.subjectMolecular dynamicsen_US
dc.subjectMembrane selectivityen_US
dc.titleInsight into membrane selectivity of linear and branched polyethylenimines and their potential as biocides for advanced wound dressingsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.schoolLee Kong Chian School of Medicine
dc.description.versionAccepted versionen_US

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