dc.contributor.authorLu, Xinyi
dc.contributor.authorGöke, Jonathan
dc.contributor.authorSachs, Friedrich
dc.contributor.authorJacques, Pierre-Étienne
dc.contributor.authorLiang, Hongqing
dc.contributor.authorFeng, Bo
dc.contributor.authorBourque, Guillaume
dc.contributor.authorBubulya, Paula A.
dc.contributor.authorNg, Huck-Hui
dc.identifier.citationLu, X., Göke, J., Sachs, F., Jacques, P.-É., Liang, H., Feng, B., et al. (2013). SON connects the splicing-regulatory network with pluripotency in human embryonic stem cells. Nature Cell Biology, 15(10), 1141-1152.en_US
dc.description.abstractHuman embryonic stem cells (hESCs) harbour the ability to undergo lineage-specific differentiation into clinically relevant cell types. Transcription factors and epigenetic modifiers are known to play important roles in the maintenance of pluripotency of hESCs. However, little is known about regulation of pluripotency through splicing. In this study, we identify the spliceosome-associated factor SON as a factor essential for the maintenance of hESCs. Depletion of SON in hESCs results in the loss of pluripotency and cell death. Using genome-wide RNA profiling, we identified transcripts that are regulated by SON. Importantly, we confirmed that SON regulates the proper splicing of transcripts encoding for pluripotency regulators such as OCT4, PRDM14, E4F1 and MED24. Furthermore, we show that SON is bound to these transcripts in vivo. In summary, we connect a splicing-regulatory network for accurate transcript production to the maintenance of pluripotency and self-renewal of hESCs.en_US
dc.description.sponsorshipASTAR (Agency for Sci., Tech. and Research, S’pore)en_US
dc.format.extent24 p.en_US
dc.relation.ispartofseriesNature Cell Biologyen_US
dc.rights© 2013 Macmillan Publishers Limited.en_US
dc.subjectEmbryonic stem cellsen_US
dc.subjectRNA splicingen_US
dc.titleSON connects the splicing-regulatory network with pluripotency in human embryonic stem cellsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US

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