The fetal mouse metatarsal bone explant as a model of angiogenesis
Fhu, Chee Wai
Ang, Koon Hwee
Liu, Cheng Hao
Johari, Nurul Azizah Binte
Moss, Stephen E
Date of Issue2015
Lee Kong Chian School of Medicine
The mouse fetal metatarsal provides a unique tool for studying angiogenesis. In comparison with other commonly used in vitro or ex vivo angiogenesis assays, vessel outgrowth from mouse fetal metatarsals is more representative of sprouting angiogensis in vivo. It allows the analysis of blood vessel growth, and the mechanisms underpinning this process, in a multicellular microenvironment that drives the formation of a robust and complex vascular network in the absence of exogenous growth factors. By labeling different constituents of the vascular structure, it is possible to perform 3D rendering of the spatial interplay between different cellular components and to carry out quantitative analysis of vessel outgrowth. High-resolution imaging permits the visualization of fine structural and cellular details. As the assay involves the use of fetal tissues, it is possible to follow new blood vessel formation in genetically modified mice that are perinatally lethal. The entire process takes 9–13 d. A detailed description of how to set up and perform the assay is described here.
Cell and Tissue Culture
© 2015 Macmillan Publishers Limited, part of Springer Nature. This is the author created version of a work that has been peer reviewed and accepted for publication by Nature Protocols, Macmillan Publishers Limited. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1038/nprot.2015.097].