Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/83373
Title: Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing
Authors: Gilmartin, Daniel J.
Soon, Allyson
Thrasivoulou, Christopher
Phillips, Anthony R. J.
Jayasinghe, Suwan N.
Becker, David Laurence
Keywords: Tissue Repair
Oligonucleotide Delivery
Issue Date: 2016
Source: Gilmartin, D. J., Soon, A., Thrasivoulou, C., Phillips, A. R. J., Jayasinghe, S. N., & Becker, D. L. (2016). Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing. Advanced Healthcare Materials, 5(14), 1786-1799.
Series/Report no.: Advanced Healthcare Materials
Abstract: Antisense oligodeoxynucleotides targeting the mRNA of the gap junction protein Cx43 promote tissue repair in a variety of different wounds. Delivery of the antisense drug has most often been achieved by a thermoreversible hydrogel, Pluronic F-127, which is very effective in the short term but does not allow for sustained delivery over several days. For chronic wounds that take a long time to heal, repeated dosing with the drug may be desirable but is not always compatible with conventional treatments such as the weekly changing of compression bandages on venous leg ulcers. Here the coating of collagen scaffolds with antisense oligonucleotides is investigated and a way to provide protection of the oligodeoxynucleotide drug is found in conjunction with sustained release over a 7 d period. This approach significantly reduces the normal foreign body reaction to the scaffold, which induces an increase of Cx43 protein and an inhibition of healing. As a result of the antisense integration into the scaffold, inflammation is reduced with the rate of wound healing and contracture is significantly improved. This coated scaffold approach may be very useful for treating venous leg ulcers and also for providing a sustained release of any other types of oligonucleotide drugs that are being developed.
URI: https://hdl.handle.net/10356/83373
http://hdl.handle.net/10220/41421
ISSN: 2192-2640
DOI: 10.1002/adhm.201600175
Rights: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Advanced Healthcare Materials, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/adhm.201600175].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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