dc.contributor.authorMilbreta, Ulla
dc.contributor.authorNguyen, Lan Huong
dc.contributor.authorDiao, Huajia
dc.contributor.authorLin, Junquan
dc.contributor.authorWu, Wutian
dc.contributor.authorSun, Chun-Yang
dc.contributor.authorWang, Jun
dc.contributor.authorChew, Sing Yian
dc.date.accessioned2016-11-02T06:36:23Z
dc.date.available2016-11-02T06:36:23Z
dc.date.issued2016
dc.identifier.citationMilbreta, U., Nguyen, L. H., Diao, H., Lin, J., Wu, W., Sun, C. Y., Wang, J., & Chew, S. Y. (2016). Three-Dimensional Nanofiber Hybrid Scaffold Directs and Enhances Axonal Regeneration after Spinal Cord Injury. ACS Biomaterials Science & Engineering, 2(8), 1319-1329.en_US
dc.identifier.urihttp://hdl.handle.net/10220/41604
dc.description.abstractSpinal cord injuries (SCIs) are followed by a complex series of events that contribute to the failure of regeneration. To date, there is no robust treatment that can restore the injury-induced loss of function. Since damaged spinal axons do not spontaneously regenerate in their native inhibitory microenvironment, a combined application of biomaterials and neurotrophic factors that induce nerve regeneration emerges as an attractive treatment for SCIs. In this study, we report the novel use of a three-dimensional (3D) hybrid scaffold to provide contact guidance for regrowth of axons in vivo. The scaffold comprises 3D aligned sparsely distributed poly(ε-caprolactone-co-ethyl ethylene phosphate) nanofibers that are supported and dispersed within a collagen hydrogel. Neurotrophin-3 was incorporated into the scaffold as an additional biochemical signal. To evaluate the efficacy of the scaffold in supporting nerve regeneration after SCIs, the construct was implanted into an incision injury, which was created at level C5 in the rat spinal cord. After 3 months of implantation, scaffolds with NT-3 incorporation showed the highest average neurite length (391.9 ± 12.9 μm, p ≤ 0.001) as compared to all the other experimental groups. In addition, these regenerated axons formed along the direction of the aligned nanofibers, regardless of their orientation. Moreover, the presence of the hybrid scaffolds did not affect tissue scarring and inflammatory reaction. Taken together, these findings demonstrate that our scaffold design can serve as a potential platform to support axonal regeneration following SCIs.en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.format.extent43 p.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesACS Biomaterials Science & Engineeringen_US
dc.rights© 2016 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by ACS Biomaterials Science & Engineering, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1021/acsbiomaterials.6b00248.en_US
dc.subjectCollagenen_US
dc.subjectElectrospinningen_US
dc.subjectNeural tissue engineeringen_US
dc.titleThree-Dimensional Nanofiber Hybrid Scaffold Directs and Enhances Axonal Regeneration after Spinal Cord Injuryen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolLee Kong Chian School of Medicine
dc.identifier.doihttp://dx.doi.org/10.1021/acsbiomaterials.6b00248
dc.description.versionAccepted versionen_US


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