dc.contributor.authorPandey, Kishor
dc.contributor.authorFerreira, Pedro Eduardo
dc.contributor.authorIshikawa, Takeshi
dc.contributor.authorNagai, Takeharu
dc.contributor.authorKaneko, Osamu
dc.contributor.authorYahata, Kazuhide
dc.date.accessioned2016-12-23T08:23:22Z
dc.date.available2016-12-23T08:23:22Z
dc.date.issued2016
dc.identifier.citationPandey, K., Ferreira, P. E., Ishikawa, T., Nagai, T., Kaneko, O., & Yahata, K. (2016). Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor. Scientific Reports, 6, 23454-.en_US
dc.identifier.issn2045-2322en_US
dc.identifier.urihttp://hdl.handle.net/10220/41947
dc.description.abstractCalcium (Ca2+)-mediated signaling is a conserved mechanism in eukaryotes, including the human malaria parasite, Plasmodium falciparum. Due to its small size (<10 μm) measurement of intracellular Ca2+ in Plasmodium is technically challenging, and thus Ca2+ regulation in this human pathogen is not well understood. Here we analyze Ca2+ homeostasis via a new approach using transgenic P. falciparum expressing the Ca2+ sensor yellow cameleon (YC)-Nano. We found that cytosolic Ca2+ concentration is maintained at low levels only during the intraerythrocytic trophozoite stage (30 nM), and is increased in the other blood stages (>300 nM). We determined that the mammalian SERCA inhibitor thapsigargin and antimalarial dihydroartemisinin did not perturb SERCA activity. The change of the cytosolic Ca2+ level in P. falciparum was additionally detectable by flow cytometry. Thus, we propose that the developed YC-Nano-based system is useful to study Ca2+ signaling in P. falciparum and is applicable for drug screening.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesScientific Reportsen_US
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
dc.subjectDrug screeningen_US
dc.subjectParasite biologyen_US
dc.titleCa2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensoren_US
dc.typeJournal Article
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doihttp://dx.doi.org/10.1038/srep23454
dc.description.versionPublished versionen_US


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