dc.contributor.authorIrvine, Scott Alexander
dc.contributor.authorSteele, Terry W. J.
dc.contributor.authorBhuthalingam, Ramya
dc.contributor.authorLi, Min
dc.contributor.authorBoujday, Souhir
dc.contributor.authorPrawirasatya, Melissa
dc.contributor.authorNeoh, Koon Gee
dc.contributor.authorBoey, Freddy Yin Chiang
dc.contributor.authorVenkatraman, Subbu S.
dc.identifier.citationIrvine, S. A., Steele, T. W. J., Bhuthalingam, R., Li, M., Boujday, S., Prawirasatya, M., et al. (2015). Quantification of aldehyde terminated heparin by SEC-MALLS-UV for the surface functionalization of polycaprolactone biomaterials. Colloids and Surfaces B: Biointerfaces, 132, 253-263.en_US
dc.description.abstractA straight forward strategy of heparin surface grafting employs a terminal reactive-aldehyde group introduced through nitrous acid depolymerization. An advanced method that allows simultaneously monitoring of both heparin molar mass and monomer/aldehyde ratio by size exclusion chromatography, multi-angle laser light scattering and UV-absorbance (SEC-MALLS–UV) has been developed to improve upon heparin surface grafting. Advancements over older methods allow quantitative characterization by direct (aldehyde absorbance) and indirect (Schiff-based absorbance) evaluation of terminal functional aldehydes. The indirect quantitation of functional aldehydes through labeling with aniline (and the formation of a Schiff-base) allows independent quantitation of both polymer mass and terminal functional groups with the applicable UV mass extinction coefficients determined. The protocol was subsequently used to synthesize an optimized heparin-aldehyde that had minimal polydispersity (PDI < 2) and high reaction yields (yield >60% by mass). The 8 kDa weight averaged molar mass heparin-aldehyde was then grafted on polycaprolactone (PCL), a common implant material. This optimized heparin-aldehyde retained its antithrombin activity, assessed in freshly drawn blood or surface immobilized on PCL films. Anticoagulant activity was equal to or better than the 24 kDa unmodified heparin it was fragmented from.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.format.extent32 p.en_US
dc.relation.ispartofseriesColloids and Surfaces B: Biointerfacesen_US
dc.rights© 2015 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Colloids and Surfaces B: Biointerfaces, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.colsurfb.2015.05.023].en_US
dc.titleQuantification of aldehyde terminated heparin by SEC-MALLS-UV for the surface functionalization of polycaprolactone biomaterialsen_US
dc.typeJournal Article
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.description.versionAccepted versionen_US

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